基础医学与临床2017，Vol.37Issue(8)：1133-1139,7.
PER2对人口腔鳞癌SCC15细胞增殖、凋亡以及生物钟基因表达的影响
Effects of PER2 on proliferation, apoptosis and clockgene expressions in human oral squamous cell carcinoma SCC15 cells
敖奕然 1赵钦 1杨凯 1郑刚2
作者信息
- 1. 重庆医科大学附属第一医院 口腔颌面外科,重庆 400016
- 2. 重庆市中医研究院,重庆 400021
- 折叠
摘要
Abstract
Objective To find the effect of alter-expressed PER2 on proliferation,apoptosis and other clock genes expression in human oral squamous cell carcinoma SCC15 cells.Methods Short hairpin RNA interference was used to knockdown PER2 in SCC15 human oral squamous cell carcinoma cells.Flow cytometry analysis was used to testify the cell proliferation and apoptosis.Quantitative real-time PCR was used to testify the mRNA expressions of PER3,BMAL1,DEC1,DEC2,CRY2,TIM,RORα,NPAS2,PER1 and REV-ERBα.Results The proliferation was enhanced and apoptosis was decreased after PER2 knockdown in SCC15 cells (P<0.05).The mRNA expression of PER3,BMAL1,DEC1,DEC2,CRY2,TIM,RORα and NPAS2 was significantly down-regulated,and the mRNA expression of PER1 and REV-ERBα was significantly up-regulated (P<0.05).Conclusions Clock gene PER2 plays an important role in regulating other clock genes of the clock gene network in cancer cells,PER2 knockdown can enhance proliferation and recede apoptosis of cancer cell.关键词
癌/口腔/生物钟基因/PER2Key words
cancer/oral cavity/clock gene/PER2分类
医药卫生引用本文复制引用
敖奕然,赵钦,杨凯,郑刚..PER2对人口腔鳞癌SCC15细胞增殖、凋亡以及生物钟基因表达的影响[J].基础医学与临床,2017,37(8):1133-1139,7.
