寄生虫与医学昆虫学报2017，Vol.24Issue(2)：109-117,9.DOI:10.3969/j.issn.1005-0507.2017.02.007
云南省西南边境登革2型病毒全基因组序列特征研究
MOLECULAR CHARACTERISTICS OF THE FULL-LENGTH GENOME OF DENGUE SEROTYPE 2 VIRUS STRAINS ISOLATED FROM DENGUE FEVER CASES IN SOUTHWESTERN BORDER REGION OF YUNNAN PROVINCE, CHINA
摘要
Abstract
To understand the molecular characteristics of the full-length genome of dengue serotype 2 virus (DENV-2) strains isolated in southwestern border region of Yunnan province,China,in 2013 and 2015 and their epidemiological feature.Dengue virus was cultured in C6/36 cell line.Viral RNA was extracted from isolates,then the full-length genome was amplified by RT-PCR.The homology and phylogenetic analyses were made on the nucleotide sequences by bioinformatics softwares including ClastalX1.83 and MEGA6 etc.Ten strains of DENV-2 were harvested from dengue fever cases,of these,6 strains from Ruili city of Dehong prefecture and 4 from Jinghong city of Xishuangbanna prefecture,Yunnan province.Phylogenetic tree and homology analysis based on structural and non-structural protein genes showed that 5 strains of the experimental virus belonged to Asian I genotype (AG-I) and the other 5 strains belonged to Cosmopolitan genotype (CG).Among them,the AG-I strains were isolated from Ruili in 2013 and 2015,and CG strains were isolated from Jinghong in 2015 as well as they have a closer genetic relationship with the strains isolated from Southeast Asia.Compared with prototype strain (New Guinea-C) of DENV-2,E and NS5 nucleotide homology of the Yunnan strains were 93.38%~93.77% and 92.80%~94.74% respectively.Yunnan strains compared with US_Hawaii strain,there were 44 and 150 different sites in amino acid of structural and non-structural proteins respectively.Only 29 and 86 different sites in amino acid of structural and non-structural proteins were found when compared to New Guinea-C strain,respectively.DENV-2 AG-I and CG transmitted in southwestern border region of Yunnan and AG-I was prevalent in Ruili in 2013 and 2015,and CG was prevalent in Jinghong in 2015.They have genetic diversity but multiple transmission sources were from neighboring countries of Southeastern Asia.It is obvious different in amino acid sites of structural and non-structural proteins between Yunnan strains and New Guinea-C strain.Their main amino acid sites for antigenicity,pathogenic and virulence of both AG-I and CG genotypes were shown without significant difference.关键词
登革2型病毒/全基因组/系统进化分析/同源性分析/氨基酸位点分析Key words
Dengue serotype 2 virus/Full length genome/Phylogenetic analysis/Homology analysis/Amino acid analysis引用本文复制引用
胡挺松,张富强,范泉水,张海林,范建华,邓波,刘永华,徐松淼,李鸿斌,尹小雄,朱进..云南省西南边境登革2型病毒全基因组序列特征研究[J].寄生虫与医学昆虫学报,2017,24(2):109-117,9.基金项目
国家十三五重大专项(2016YFC1200100) （2016YFC1200100）
中国博士后基金项目(2015M582907 （）
2016T91009) （）
