扬州大学学报（农业与生命科学版）2017，Vol.38Issue(2)：1-4,9,5.DOI:10.16872/j.cnki.1671-4652.2017.02.001
布鲁菌抗原蛋白的原核表达、纯化与鉴定
Prokaryotic expression, purification and identification of Brucella antigenic proteins
摘要
Abstract
The target fragments of rplL,groEL,bp26,omp25,p39 and sod1 gene of B.abortus S19 with expected size of 375,1 641,753,642,1 206 and 522 bp were amplified by PCR,and those genes were cloned into pMD 20-T and pET-28a or pCold I vector,respectively.The recombinant plasmids were transformed into E.coli BL21 (DE3),and the recombinant proteins were induced by IPTG and analyzed by SDS-PAGE and Western blot.Results of SDS-PAGE showed that the fusion proteins were expressed successfully with molecular weight about 20,65,33,28,47 and 24 ku,respectively.Western of blot analysis showed that these recombinant proteins could react with anti-His MAb specifically,which further confirmed the expression of these proteins.关键词
布鲁菌/原核表达/重组蛋白Key words
Brucella/prokaryotic expression/recombinant protein分类
农业科技引用本文复制引用
徐正中,杜强,孟闯,李昕,夏爱鸿,何晶晶,潘志明,陈祥,焦新安..布鲁菌抗原蛋白的原核表达、纯化与鉴定[J].扬州大学学报（农业与生命科学版）,2017,38(2):1-4,9,5.基金项目
国家自然科学基金资助项目(31602031) （31602031）
江苏省重点研发计划项目(BE2015343) （BE2015343）
江苏省自然科学基金资助项目(BK20160466) （BK20160466）
江苏高校青蓝工程项目(2016-05) （2016-05）
江苏高校优势学科建设工程项目(2014-05) （2014-05）
