扬州大学学报（农业与生命科学版）2017，Vol.38Issue(2)：5-9,5.DOI:10.16872/j.cnki.1671-4652.2017.02.002
奶牛源葡萄球菌tetK基因碱基及氨基酸序列差异的功能分析
Functional analysis of tetK gene base and its amino acid sequence difference in Staphylococcus isolated from cows
摘要
Abstract
The tetK gene of Staphylococcus aureus isolated from cows plays an important role in tetracycline resistance.The S.aureus strains MP00109 and MP00159 were sensitive and resistance to tetracycline respectively.The tetK′and tetK genes of MP00109 and MP00159 were amplified by PCR.Then the competent BL21 (DE3) cells were transformed with the expression plasmids of tetK′ and tetK respectively.Compared with the sequences of tetK′ and tetK,five nucleotides and four amino acids were different.The BL21 transformed with tetK′ was sensitive to tetracycline while the BL21 transformed with tetK was resistant to tetracycline.Thus,we speculate the mutation of partial nucleotide positions in tetK gene causes the tetracycline-resistance change in Staphylococcus aureus.关键词
奶牛源葡萄球菌/四环素耐药基因tetK/耐药表型/基因突变Key words
cow-derived Staphylococcus isolates/tetracycline resistance gene tetK/resistant phenotype/gene mutation分类
农业科技引用本文复制引用
冯清,秦爱建,金文杰,林伟东,张大帅,黄攀,戴迪,廖俊,梁祥焕,王建,张军..奶牛源葡萄球菌tetK基因碱基及氨基酸序列差异的功能分析[J].扬州大学学报（农业与生命科学版）,2017,38(2):5-9,5.基金项目
江苏省青蓝工程项目(2012-05) （2012-05）
扬州市社会发展科技攻关项目(YZ2011091) （YZ2011091）
江苏省农业科技自主创新资金项目[CX(14)2088] （14）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
常州市科技支撑计划项目(CE20152006) （CE20152006）
