扬州大学学报（农业与生命科学版）2017，Vol.38Issue(2)：15-20,6.DOI:10.16872/j.cnki.1671-4652.2017.02.004
CDNF-BMSCs移植治疗帕金森病的试验研究
Experimental study on therapeutic effect of CDNF gene modified bone mesenchymal stem cells transplantation in PD model rats
摘要
Abstract
In this study,eukaryotic expression plasmid pcDNA3.1(+)-CDNF(cerebral dopamine neurotrophic factor)was recombined to bone mesenchymal stem cell (BMSCs) by the Lipo 2000 for establishing CDNF-BMSCs.CDNF-BMSCs were transplanted into the striate body of the PD model rats for the therapy by brain stereotaxic technique.Rotating behavior was tested in different periods after treatment respectively.Immunohistochemical and WB technology were took to detect the expression of CDNF and tyrosine hydroxylase (TH) in the striate body for exploring the effect of the CDNFBMSCs on Parkinson's disease(PD).The result showed that the behavior of CDNF-BMSCs group was improved significantly after two weeks from transplantation treatment.CDNF protein expression was very significantly increased and TH protein expression was increased significantly.At the same time,the CDNF and TH positive cells in the striate body of CDNF-BMSCs group increased gradually over time.Therefore,this study provided an experimental evidence for the gene therapy of neurodegenerative diseases,such as Parkinson's disease.关键词
大鼠/帕金森病/骨髓间充质干细胞/大脑多巴胺能神经营养因子/基因治疗Key words
rat/Parkinson's disease/bone mesenchymal stem cell/cerebral dopamine neurotrophic factor/gene therapy分类
医药卫生引用本文复制引用
陈雨松,薛莹,潘士锋,董轩,邢华..CDNF-BMSCs移植治疗帕金森病的试验研究[J].扬州大学学报（农业与生命科学版）,2017,38(2):15-20,6.基金项目
国家自然科学基金资助项目(31172284) （31172284）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
