扬州大学学报（农业与生命科学版）2017，Vol.38Issue(2)：47-51,5.DOI:10.16872/j.cnki.1671-4652.2017.02.009
基于mtDNA D-loop区全序列的琅琊鸡遗传多样性和系统进化研究
The analysis on the genetic diversity and phyletic evolution of Langya chicken based on mitochondrial DNA D-loop region sequence
摘要
Abstract
To investigate the genetic diversity and phyletic evolution,realize the maternal origin of Langya chicken,30 Langya chickens were used as material,by PCR amplification and sequencing technique,complete mtDNA D-loop sequences of Langya chickens were analyzed,and compared to the 19 red jungle fowl sequences in GenBank.The results showed that,the D-loop region of Langya chicken was 1 231-1 232 bp,the 1 231 bp haplotype existed a base C deficiency in 859 site.In the 30 samples assayed,25 mutation sites and 13 haplotypes were found.The nucleotide diversity (Pi) was 0.006 47±0.005 13,haplotype diversity (Hd) was 0.887±0.038,and average number of nucleotide differences (K) was 7.968.Phylogenetic analysis showed that Langya chickens might have multiple origins.13 haplotypes of Langya chicken converged into 4 clades of A,B,C and E.The clade E was the advantage haplotype,which clustered with Gallus gallus murghi.The results will be a reference for genetic resources conservation and development and utilization of Langya chicken.关键词
琅琊鸡/mtDNA/D-loop区/遗传多样性/系统进化Key words
Langya chicken/mitochondrial DNA/D-loop region/genetic diversity/phyletic evolution分类
农业科技引用本文复制引用
唐修君,贾晓旭,樊艳凤,高玉时,葛庆联,顾荣,陆俊贤,刘茵茵,王珏..基于mtDNA D-loop区全序列的琅琊鸡遗传多样性和系统进化研究[J].扬州大学学报（农业与生命科学版）,2017,38(2):47-51,5.基金项目
国家自然科学基金资助项目(31372277、31501917) （31372277、31501917）
