解放军医学院学报2017，Vol.38Issue(7)：662-667,679,7.DOI:10.3969/j.issn.2095-5227.2017.07.017
幽门螺杆菌感染对葡聚糖硫酸钠诱导的小鼠慢性结肠炎的保护作用
Effect of Helicobacter pylori infection on dextran sulfate sulphate induced chronic colitis in mice
李璟 1王蔚虹 1张鸿晨 1戴芸 1刘芸1
作者信息
- 1. 北京大学第一医院消化内科,北京100034
- 折叠
摘要
Abstract
Objective To investigate the effect of Helicobacter pylori (H.pylori) infection on dextran sulfate sulphate (DSS)-induced chronic colitis in mice,and explore its potential mechanism.Methods Chronic colitis was induced by three 5-day cycles of 2％ DSS in C57BL/6J mice infected with H.pylori.Forty mice were randomly divided into 4 groups:normal control group,H.pylori infection group (HP group),DSS induced colitis group (DSS group),and DSS induced colitis with H.pylori infection group (HP+DSS group).The disease activity index (DAI),colon length and histopathologic scores of colitis in DSS group and HP+DSS group were evaluated.The degree of infiltration of CD3+ T lymphocytes was evaluated by immunohistochemical staining,and Th17 and Treg subsets in lamina propria were detected by flow cytometry.Results The amounts of CD3+T lymphocyte infiltration in colon of control group and HP group were (10.83 ± 3.97)/HPF vs (30.67 ± 7.37)/HPF,respectively (P ＜ 0.01).Compared with the DSS group,DAI (P ＜ 0.05),shortening of colon length [(6.84 ± 0.64) cm vs (8.14 ± 0.42) cm,P ＜ 0.01],histopathologic score [(9.50 ± 1.38) vs (6.83 ± 1.72),P ＜ 0.05] and infiltration of CD3+T cells in colon [(205.17 ± 32.28)/HPF vs (125.17 ± 15.66)/HPF,P ＜ 0.01] decreased more significantly in HP+DSS group.H.pylori infection decreased Thl7 subsets in lamina propria of HP+DSS group [(4.56％ ± 0.34)％ vs (2.09％ ± 0.35)％,P ＜ 0.05],while it increased Treg subsets [(2.01％ ± 0.30)％ vs (6.46％ ± 0.33)％,P ＜ 0.05] as compared with DSS group.Conclusion H.pylori infection has a protective effect on DSS-induced chronic colitis in mice.The immune response mediated by Thl7 and Treg in colon may be involved in the process.关键词
幽门螺杆菌/慢性结肠炎/细胞免疫Key words
Helicobacterpylori/chronic colitis/cellular immunity分类
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李璟,王蔚虹,张鸿晨,戴芸,刘芸..幽门螺杆菌感染对葡聚糖硫酸钠诱导的小鼠慢性结肠炎的保护作用[J].解放军医学院学报,2017,38(7):662-667,679,7.
