解放军医学院学报2017，Vol.38Issue(7)：686-688,692,4.DOI:10.3969/j.issn.2095-5227.2017.07.022
环境致癌物苯并[a]芘调控原癌基因c-jun的机制研究
Benzopyrene affected on the pathogenesis of lung cancer by up-regulating c-jun
封琳 1刘晓婷 2宋欣1
作者信息
- 1. 解放军总医院病理科,北京100853
- 2. 解放军总医院临检科,北京100853
- 折叠
摘要
Abstract
Objective To explore the mechanism of environmental carcinogen benzopyrene promoting the pathogenesis of lung cancer.Methods Human bronchial epithelium (HBE) cells and non-small cell lung cancer cell line H1299 were treated with benzopyrene (Bap) by different exposure doses (0 μmol/L,2.5 μmol/L,5 μmol/L,10 μmol/L),different exposure time (0 h,6 h,12 h,24 h) and the presence or absence of BaP receptor AhR inhibitor α-NF,and the expressions of c-jun protein under the different conditions were evaluated.Results With the increase of BaP dose,the relative expressions of c-jun protein in HBE and H1299 were all increased from 0.04 (dose 0 μ mol/L) to 0.84 (dose 10 μ mol/L) (P ＜ 0.01) and 0.91 (dose 0 μ mol/L) to 1.3 (dose 10 μmol/L) (P ＜ 0.01);with the increase of exposure time,the relative expressions of c-jun protein of HBE and H1299 were up-regulated from 0.08 (exposure time 0 h) to 0.87 (exposure time 24 h) (P ＜ 0.01) and from 0.33 (exposure time 0 h) 0.58 (exposure time 24 h) (P ＜ 0.05).The expression of c-jun protein was positively related to the dose and exposure time of BaP.After adding AhR inhibitor α-NF,the expression of c-jun protein in the nucleus decreased from 2.1 to 0.7 (P ＜ 0.05) in HBE cells.Conclusion In HBE and H1299 cell lines,the environmental carcinogen benzopyrene induced the malignant transformation of the cells by up-regulating the expression of proto-oncogene c-jun in a dose-department and time-department manner.关键词
苯并(a)芘/原癌基因c-jun/肺癌Key words
benzo (a) pyrene/c-jun/lung cancer分类
医药卫生引用本文复制引用
封琳,刘晓婷,宋欣..环境致癌物苯并[a]芘调控原癌基因c-jun的机制研究[J].解放军医学院学报,2017,38(7):686-688,692,4.
