检验医学与临床2017，Vol.14Issue(15)：2264-2265,2324,3.DOI:10.3969/j.issn.1672-9455.2017.15.034
生殖道支原体对米诺环素的耐药性及相关耐药基因分析
Resistance of genital Mycoplasma to minocyline and the related resistant gene
谢明水 1刘杨1
作者信息
- 1. 湖北医药学院附属随州医院检验科 441300
- 折叠
摘要
Abstract
Objective To explore the resistance of genital Mycoplasma to minocycline and the distribution of related resistant gene.Methods Genital Mycoplasma was isolated from 1 076 genital tract infections.Immu-Mark Myco-Test Kit was used to identify Mycoplasma and their sensibilities to tetracyclines were dectected.Then positive rate of Mycoplasma with minocycline-resistance gene TetM through Kit was investigated.Results A total of 387 strains of Mycoplasma positive strains were isolated,UU strains were accounted for 71.06%,MH strains were accounted for 5.43%,UU+MH strains were accounted for 23.51%.With the increase of the concentration of minocycline,strain tolerance was decreased gradually,when minocycline concentration was 1.000 0 mg/L,the antibacterial effect was the best.The detection rate of TetM gene in intermediate strains was significantly higher than that of the susceptible strain(P<0.05).The positive detection rate of TetM in the drug resistant strains was significantly higher than that of the intermediate strains and the sensitive strains(P<0.05).Conclusion Genital Mycoplasma strains have shown varying degrees of tetracycline minocycline resistance.Tetracycline-resistance of strains is closely related to TetM gene,which could be an important reference to assess the tetracycline resistance of Mycoplasma infections.关键词
人型支原体/解脲支原体/米诺环素/TetMKey words
Mycoplasma hominis/Ureaplasma urealyticum/tetracycline/TetM引用本文复制引用
谢明水,刘杨..生殖道支原体对米诺环素的耐药性及相关耐药基因分析[J].检验医学与临床,2017,14(15):2264-2265,2324,3.
