临床输血与检验2017，Vol.19Issue(4)：317-320,4.DOI:10.3969/j.issn.1671-2587.2017.04.002
CD4+CD25+FOXP3+调节性T细胞在哮喘小鼠发病中的作用
CD4+CD25+FOXP3+ Regulatory T cells in Asthmatic Mice
摘要
Abstract
Objective To observe the change of CD4+CD25+FOXP3+ regulatory T cells (Tregs) in mouse model of asthma.Methods A total of 20 Balb/c female SPF-class mice were randomly divided into normal control group (n=10) and asthma model group (n=10).The asthma model group was sensitized with 2% ovalbumin (OVA) by a combination of intraperitoneal injection to induce asthma.Mice of normal control group received normal salineinstead of OVAin equal volume.The counts of eosinophils in peripheral blood (PB)and bronchoalveolar lavage fluid (BALF) weredetected.The number of Tregs in mouse spleens was analyzed.Results The numbers of CD4+CD25+/CD4+(%), CD4+FOXP3+/CD4+(%),and CD4+CD25+FOXP3+/CD4+(%) in mouse spleens in asthma and normal control groups were 2.50±0.12 vs.16.64±2.11,3.92±1.23 vs.27.72±0.15,and 1.34±0.25 vs.15.60±0.62,respectively,showing significant differences (P<0.05).Conclusion The proportion of CD4+CD25+FOXP3+ cells in asthma mice was decreased compared to controls,suggesting that tregsmay be involved in asthma pathogenesis.关键词
哮喘/调节性T细胞/FOXP3Key words
Asthma/Regulatory T Cell/FOXP3分类
医药卫生引用本文复制引用
杨慧慧,张衡,苏虹..CD4+CD25+FOXP3+调节性T细胞在哮喘小鼠发病中的作用[J].临床输血与检验,2017,19(4):317-320,4.基金项目
本课题受安徽省自然科学基金(No.1408085MH159)资助 （No.1408085MH159）
