临床输血与检验2017，Vol.19Issue(4)：358-361,4.DOI:10.3969/j.issn.1671-2587.2017.04.014
丙型肝炎病毒抗体复查策略的研究
Study on the Rechecked Strategy of Antibody to Hepatitis C Virus
张菁 1邵春燕 1卓海龙 1王海平 1骆群1
作者信息
- 1. 100071 中国人民解放军第三〇七医院输血科
- 折叠
摘要
Abstract
Objective To Investigate the rechecked strategy of chemiluminescence immunoassay(CLIA) combined with enzyme linked immunosorbent assay(ELISA)in order to detect Hepatitis C virus antibodies. Methods Two hundred and one sera samples of CLIA anti-HCV S/CO >0.40 were collected from the patients by ELISA. and the samples over the cutoff value with at least one reagent detection were checked for HCV RNA by realtime fluorescence quantitative PCR(FQ-PCR) . Results Among the positive 201 samples,CLIA provided by Johnson,ELISA by MUREX,and ELISA by Kehua companies revealed positivities of 2.5%,1.6%,and 1.5%,respectively. There were 75 (1.3%,75/5800)samples that had both CLIA and two ELISA reagents positive detections. FQ-PCR positive detections were noted in 51(0.9%,51/5800)samples. Forty samples exhibited anti-HCV S/CO>0.40 and <0.90 in CLIA detection. Eight samples showed anti-HCV S/CO>0.90 and <0.99 in CLIA detection. There were 153 samples that revealed the anti-HCV S/CO ≥1.0 in CLIA detection,among them,66 samples indicated S/CO 1.00-5.00,16 samples,S/CO 5.01-10.00;5 samples,S/CO 10.01-20.00; and 66 samples,S/CO >20.00. Conclusion CLIA is reliable for detection of anti- HCV with S/CO>0.40 and <0.90. HCV RNA detection is useful for laboratory diagnosis and treatment evaluation in the samples that have showed positive anti-HCV by using CLIA and ELISA.关键词
化学发光法/酶联免疫法/丙型肝炎病毒抗体Key words
Chemiluminescence immunoassay/Enzyme linked immunosorbent assay/Hepatitis C virus antibody分类
医药卫生引用本文复制引用
张菁,邵春燕,卓海龙,王海平,骆群..丙型肝炎病毒抗体复查策略的研究[J].临床输血与检验,2017,19(4):358-361,4.
