临床与实验病理学杂志2017，Vol.33Issue(6)：591-595,5.DOI:10.13315/j.cnki.cjcep.2017.06.001
胃癌组织microRNA表达谱检测及生物信息学分析
Expression profile of microRNAs and bioinformatics analysis in human gastric cancer tissues
摘要
Abstract
This experiment was designed to investigate the expression profile of miRNAs (microRNA) in human gastric cancer tissues.Methods The expression profiles of miRNAs were compared between 3 pairs of GC and adjacent normal tissues using an Exiqon miRNA array,following which quantitative PCR (qPCR) was employed to confirm the results of the miRNA array,and 10 miRNAs were selected.Bioinformatics was used to analyze the biological function of the differentially expressed miRNAs and its target genes in gastric cancer.Results Compared with adjacent mucosal tissues,95 miRNAs were up-regulated and 16 miRNAs were down-regulated in GC (＞ 2 folds,P ＜ 0.05).The qPCR results were consistent with microarray-based expression analysis (P =0.049).Furthermore,the online GO and pathway analysis revealed that miRNAs might involve RNA transcription,RNA metabolism,gene expression,gene silencing,and other biological functions in GC.Conclusion There is abnormal expression of miRNAs in gastric cancer,and the abnormal expression of miRNAs may be related to GC tumorigenesis.关键词
胃肿瘤/miRNA/差异表达/生物信息学/miRNA芯片Key words
gastric neoplasms/microRNA/differential expression/bioinformatics/microRNA array分类
医药卫生引用本文复制引用
黄幼生,解娜,张艺馨,罗志飞,薛逢贵..胃癌组织microRNA表达谱检测及生物信息学分析[J].临床与实验病理学杂志,2017,33(6):591-595,5.基金项目
国家自然科学基金(81260321) （81260321）
