临床与实验病理学杂志2017，Vol.33Issue(6)：636-640,5.DOI:10.13315/j.cnki.cjcep.2017.06.009
层粘连蛋白α2亚链在膜性肾病肾小球基膜的异常分布
Abnormal distribution of laminin α2 chain in glomerular basement membrane in membranous nephropathy
摘要
Abstract
To study the abnormal distribution of type Ⅳ collagen and laminin chains in glomerular basement membrane (GBM) in membranous nephropathy (MN).Methods 52 cases of MN were collected and staged according to electron microscopic morphological characteristics,and 10 cases of kidney tissues of minimal change disease were used as normal GBM control.Distribution pattern of or5 (Ⅳ) chain,laminin α5and β2 chains,and laminin α2 and β1 chains were detected using immunofluorescence method.Results In minimal change disease,α5 (Ⅳ) chain,laminin α5 and β2 chains all showed continuously linear positive expression along GBM,and laminin α2 and β1 chains were negatively expressed in GBM.In stage Ⅰ MN,α5 (Ⅳ) chain,laminin α.5 and β2 chains all showed continuous linear positive expression along GBM.In stage ⅡMN,the expression of α5 (Ⅳ) chain was increased and showed abundant spikes on the basis of continuous linear positive staining along GBM,and the expression of laminin α5 and β2chains was increased,and segmental spikes were seen on the basis of continuous linear positive staining along GBM.In stage ⅢMN,the expression of α5 (Ⅳ),laminin α5 and β2 chains was also enhanced and segmental double tracks were seen.The expression of laminin α2 chain was negative in GBM in stage ⅠMN,but granular positive expression along GBM was seen in stage Ⅱ and stage Ⅲ MN.No positive expression of laminin β1chain was seen in GBM in different stages in MN.Conclusion The GBM thickness in MN originates not only from intrinsic type Ⅳ collagen chains and laminin chains,but also from laminin α2chain,which only exist in glomerulus mesangium in normal condition.关键词
膜性肾病/层粘连蛋白α2/肾小球基膜Key words
membranous nephropathy/laminin α2/glomerular basement membrane分类
医药卫生引用本文复制引用
杨晓青,黄岩杰,吕伟刚,李静,毕亮亮,梅晓峰,李金刚..层粘连蛋白α2亚链在膜性肾病肾小球基膜的异常分布[J].临床与实验病理学杂志,2017,33(6):636-640,5.基金项目
河南省郑州市普通科技攻关计划(153PKJGG170)、河南省科技创新杰出青年科技计划(144100510014) （153PKJGG170）
