环境与职业医学2017，Vol.34Issue(7)：636-641,6.DOI:10.13213/j.cnki.jeom.2017.16695
纳米银的银离子释放及其对细胞的毒性作用
Release of silver ions from silver nanoparticles and their cytotoxicity
摘要
Abstract
[Objective] To test the silver ion release kinetics of three kinds of silver nanoparticles under different conditions (such as mass concentration,time,and solution) and assess the cytotoxicity of released silver ions.[Methods] Uncoated 20nm,uncoated 50nm,and polyvinylpyrrolidone coated 20nm silver nanoparticles (nano-Ag20,nano-Ag50,and nano-Ag20PVP) were dispersed in different pH buffers such as pH3.6 acetate buffer,pH6.0 acetate buffer,and pH7.0 deionized water to prepare 0.2,0.5,and 1.0 g/L suspensions.After incubation at 37℃,silver ions were separated by ultracentrifugation and ultrafiltration for detection.Nano-Ag20PVP were dissolved in cell culture to prepare solutions of 0,20,40,80,and 160mg/L concentrations,then incubated at 37℃,and followed by silver ion detection after 24 and 48 h,respectively.A549 and HepG2 cells were exposed to nano-Ag20PVP suspensions,with silver nitrate solution used as positive control,to determine cell viability by MTT assay.[Results] Higher degree of dispersion was found in nano-Ag20PVP than in nano-Ag20 or nano-Ag50.In all designed pH conditions,the silver ion content of three kinds of silver nanoparticles was positively correlated with mass concentration and time.Under same pH and mass concentration conditions,the silver ion ratio of nano-Ag20PVP was always higher than that of nano-Ag20 (P＜0.05).At pH7.0,the silver ion ratio of nano-Ag50 was higher than that of nano-Ag20 (P＜0.05) in the 1.0g/L group.The silver ion ratios of the 20 mg/L and the 160 mg/L nano-Ag20PVP solutions were 0.02％ and 0.09％,respectively.After incubation for 24 h and 48 h,the silver ion ratios of the 160mg/L nano-Ag20PVP were 0.01％ and 0.02％,respectively.After treatment for 24h and 48 h with nanoAg20PVP,both silver content and silver ion ratio in A549 and HepG2 cells were positively associated with the mass concentration of nano-Ag20PVP.After 24 h with nano-Ag20PVP treatment,the content of silver in HepG2 cells was higher than that in A549 cells (P ＜ 0.05),but it was lower after 48 h (P ＜ 0.05).After treatment for 24 h and 48 h with nano-Ag20PVP,the contents of silver ion in HepG2 cells were lower than those in A549 cells (P ＜ 0.05).After treatment for 24 h and 48 h with 10-160 mg/L nano-Ag20PVP,the cell viabilities of HepG2 cells were higher than those of A549 cells (P ＜ 0.05).After treatment for 24 h and 48 h with 5 and 10 mg/L silver nitrate,the cell viabilities of HepG2 cells were higher than those of A549 cells (P ＜ 0.05).At the same mass concentration (5 and 10 mg/L),the cell viability of the nano-Ag20PVP group was higher than that of the silver nitrate group (P ＜ 0.05).[Conclusion] Coated silver nanoparticles release more silver ions than uncoated silver nanoparticles.Nano-Ag20PVP could still release silver ions in cells,which plays an important role in the cytotoxicity induced by silver ions in selected cell lines.Silver nanoparticles and released silver ions jointly induce cytotoxicity.关键词
纳米银/银离子/A549细胞/HepG2细胞/细胞毒性Key words
silver nanoparticle/silver ion/A549 cell/HepG2 cell/cytotoxicity分类
医药卫生引用本文复制引用
孙晋都,张帮勇,李婷竹,高晓洁,王君君,薛玉英,唐萌..纳米银的银离子释放及其对细胞的毒性作用[J].环境与职业医学,2017,34(7):636-641,6.基金项目
国家自然科学基金项目(编号:81573186,81502783,81473003,81302461) （编号:81573186,81502783,81473003,81302461）
