南京师大学报（自然科学版）2017，Vol.40Issue(2)：43-50,8.DOI:10.3969/j.issn.1001-4616.2017.02.008
根皮素引起前列腺癌LNCaP细胞发生凋亡的机制研究
Molecular Mechanism of Phloretin-Induced Apoptosis in Prostate Cancer LNCaP Cells
摘要
Abstract
Phloretin,a natural product found in pears,apples and various vegetables,exerts anti-inflammatory,anti-oxidant and anti-tumor effects.Here,we investigated the molecular mechanism of phloretin-induced apoptosis in prostate cancer LNCaP cells.In this study,effects of phloretin on the viability of prostate cancer cells were investigated by morphological observation,MTT assay and CCK-8 assay,respectively.Effect of phloretin on apoptosis and cell cycle was measured by Flow Cytometry.Furthermore,western blot was employed to investigate the levels of phosphorylation of PI3K and AKT (the activity of PI3K/AKT signaling pathway);and check the expression of cell apoptosis-related and cell cycle-related proteins which were downstream of PI3K/AKT signaling pathway in LNCaP cells.As a result,we found that phloretin significantly blocked cell cycle arrest in G2/M phase by decreasing the protein level of Cyclin B1,and decreased the cell viability and increased the nuclear rupture in a dose-dependent manner;and finally resulted in a significantly apoptosis in LNCaP cells.In molecular level,phloretin showed a dose-dependent down-regulation of the activity of PI3K/AKT via down-regulating the phosphorylation of PI3K and AKT proteins,and then regulated the expression of cell apoptosis-related and cell cycle-related proteins.Therefore,phloretin significantly inhibited cell viability and promoted apoptosis in prostate cancer cells,suggesting that phloretin had potential clinical value for treatment of prostate cancer in clinic.关键词
根皮素/细胞凋亡/细胞周期/PI3K/AKT信号通路/前列腺癌Key words
phloretin/cell apoptosis/cell cycle/PI3K/AKT signal pathway/prostate cancer分类
生物科学引用本文复制引用
康丹,吴朝蒙,曹润怿,王方方,田倩,刘平..根皮素引起前列腺癌LNCaP细胞发生凋亡的机制研究[J].南京师大学报（自然科学版）,2017,40(2):43-50,8.基金项目
国家自然科学基金(81272850、81472415). （81272850、81472415）
