农业机械学报2017，Vol.48Issue(7)：272-279,8.DOI:10.6041/j.issn.1000-1298.2017.07.034
基于卧式厌氧装置的稻秸高固态消化与甲烷菌变化研究
High Solid-state Digestion of Rice Straw and Changes of Methanogens in Horizontal Anaerobic Reactor
摘要
Abstract
Instability of high solid-state anaerobic digestion often happened during the long-term operation of reactor.To investigate underlying methanism of this problem,the characteristics of semi-continuous anaerobic digestion for rice straw were studied with three organic loading rates (OLR) in a horizontal reactor,when being co-inoculated with ruminal contents and anaerobic sludge.The results indicated that the highest volume biogas production reached 1.04 L/(L·d) in the reactor.The average CH4 productivity reached 280.90 mL/g VS with 54.39％ CH4 volume content at the OLR of 2.26 g/(L·d),which was up to 80.29％ of the theoretical value of rice straw.The highest digestion rates of hemicellulose and cellulose were 49.71％ and 31.25％,respectively.And the cellulase activities were increased obviously in the horizontal reactor,which was indispensable to the degradation of cellulose.At the OLR of 2.47 g/(L·d),the average concentration of ammonium nitrogen reached 1 082.63 mg/L.Especially the highest concentration reached 1 196.37 mg/L after 192 d of operation.It was indicated that the methanogens suffered different influences from ammonium nitrogen using quantitative real-time PCR (Q-PCR).Specifically,the hydrogenotrophic Methanobacteriales was decreased to 1.04 × 106 copies/g from 1.70 ×109 copies/g dominated in the solid residues and CH4 productivity was decreased to 256.54 mL/g for being inhibited significantly.In contrast,the acetotrophic Methanosarcinales was increased to 9.44 × 106 copies/g from 7.89 × 106 copies/g.At the same time,the average propionate concentration reached 253.32 mg/L in the anaerobic reactor.Thus the study revealed the adaptive shifts of underlying methanogenic communities by ammonium nitrogen inhibition during the high solid-state digestion of rice straw.关键词
卧式反应装置/稻秸/高固态消化/甲烷菌群Key words
horizontal reactor/rice straw/high solid-state digestion/methanogenic community分类
农业科技引用本文复制引用
邓玉营,阮文权,黄振兴,缪恒峰,赵明星,施万胜..基于卧式厌氧装置的稻秸高固态消化与甲烷菌变化研究[J].农业机械学报,2017,48(7):272-279,8.基金项目
国家自然科学基金项目(51678279,21506076),“十二五”国家科技支撑计划项目(2014BAC25B01)和常州工程职业技术学院校级科研基金项目(11130100116022) （51678279,21506076）
