农业生物技术学报2017，Vol.25Issue(7)：1072-1077,6.DOI:10.3969/j.issn.1674-7968.2017.07.004
ASIP-YY对羊驼皮肤黑色素细胞形态和黑色素生成的影响
Influence of ASIP-YY on Morphology and Melanogenesis of Alpaca (Lama pacos) Skin Melanocytes
摘要
Abstract
Agouti gene is one of the important influencing animal coat color genes,although the expression of Agouti gene was found in a variety of animal,Agouti protein (Agouti signaling protein,ASIP) reports was rare on effects of Alpaca (Lama pacos) proliferation of melanocytes and genes related to coat color.In order to observe effect ofASIP-YY (a C-terminal fragment of ASlP) on melanocytes and the formation of Alpaca skin melanin,the different concentrations of ASIP-YY was used in extra-organismal melanocytes of Alpaca skin.Different concentrations of ASIP-YY (0.0,0.1,1.0,10.0,100.0 nmol/L) treated melanocytes of Alpaca Skin at 72 h.The activity of melanocytes were detected using methyl thiazolyl tetrazolium (MTT) method,the melanin content was tested with ultraviolet spectrophotometric method and qRT-PCR was used to detect the expressions of TYR,TRP-1,TRP-2,MC1R and MTIF mRNA.The results showed that the melanocytes activity was inhibited;the expression of TYR mRNA was significantly inhibited by 0.1,10.0,100.0 nmol/L ASIP-YY (P＜0.01),the expression of TRP-1 mRNA was significantly inhibited by 10.0,100.0 nmol/L ASIP-YY(P＜ 0.01),the expression of MCIR mRNA was inhibited by 10.0 nmol/L ASIP-YY (P＜0.05),the expression of MITF mRNA was significantly inhibited by 0.1,1.0,10.0 nmol/L ASIP-YY(P＜0.01),0.1,1.0 nmol/L ASIP-YY promoted the expression of TRP-1,TRP-2 mRNA(P＜0.05),1.0,100.0 nmol/L ASIP-YY significantly promoted the expression of MC1R mRNA(P＜0.01).ASIP-YY can make the size of melanocytes to be smaller and inhibit the expression of melanin related gene.ASIP-YY may be competitive with MC1R to combine,so as to inhibit the expression of melanin related genes.This study provides a theoretical basis for further study on the reeulation of ASIP on Alpaca skin melanin formation.关键词
ASIP-YY/羊驼/黑色素细胞/黑色素Key words
ASIP-YY/Alpaca (Lama pacos)/Melanocytes/Melanin分类
农业科技引用本文复制引用
南瑞鹏,牛峰,李振,卢杰丽,庞全海..ASIP-YY对羊驼皮肤黑色素细胞形态和黑色素生成的影响[J].农业生物技术学报,2017,25(7):1072-1077,6.基金项目
国家自然科学基金项目(No.30972223和No.31272628) （No.30972223和No.31272628）
