农药学学报2017，Vol.19Issue(4)：457-464,8.DOI:10.16801/j.issn.1008-7303.2017.0060
Cylindrocarpon olidum W1菌株代谢物中壳二孢氯素及其类似物的分离及抑菌活性
Isolation and antimicrobial activities of ascochlorin and its analogues from the metabolites of Cylindrocarpon olidum W1
摘要
Abstract
Eight compounds W1-1–W1-8 were isolated from the secondary metabolites of Cylindrocarpon olidum W1, which is an endophytic fungus from Euonymus japonica, by silica gel column chromatography and pre-HPLC techniques. Based on the 1H NMR and 13C NMR and HRMS-MS data, as well as the published literatures, these chemicals were identified as Ilicicolin A (W1-1), 5-Chlorocolletorin B (W1-2), Ilicicolin B (W1-3), Deacetylchloronectrin (W1-4), Ilicicolin C (W1-5), Ascochlorin (W1-6), Cylindrol A4 (W1-7) and Cylindrol B (W1-8). The obtained Ascochlorin and its analogues showed strong antibacterial activities against Ralstonia solanacearum, with minimum inhibitory concentrations (MICs) of 6.25, 3.13, > 100, 3.13, 6.25, 12.5, 25 and 25 μg/mL, respectively. Compounds W1-2, W1-4 and W1-5 could also effectively inhibit the mycelial growth of plant pathogenic fungi Botrytis cinerea and Sclerotinia sclerotiorum. Their medium inhibitory concentrations (IC50) against B. cinerea were 36.45, 21.60 and 26.69 μg/mL, respectively. Their IC50 values against S. sclerotiorum were 20.21, 16.79 and 12.11 μg/mL, respectively.关键词
CylindrocarponolidumW1/壳二孢氯素/分离鉴定/抑菌活性Key words
Cylindrocarpon olidum W1/ascochlorin/isolation and identification/antimicrobial activity分类
生物科学引用本文复制引用
魏少鹏,姬志勤..Cylindrocarpon olidum W1菌株代谢物中壳二孢氯素及其类似物的分离及抑菌活性[J].农药学学报,2017,19(4):457-464,8.基金项目
国家重点研发计划 (2017YFD0200506) （2017YFD0200506）
国家自然科学基金资助项目 (31371973,31301700) （31371973,31301700）
陕西省科技攻关项目 (2016NY-139). （2016NY-139）
