山东医药2017，Vol.57Issue(29)：5-7,3.DOI:10.3969/j.issn.1002-266X.2017.29.002
索拉非尼对肝癌细胞中PD-L1表达的影响及机制
Effect of sorafenib on expression of PD-L1 in hepatocellular carcinoma cells
摘要
Abstract
Objective To investigate the effect of sorafenib on the expression of programmed cell death ligand 1(PD-L1) in the hepatocellular carcinoma cells and the underlying mechanism.Methods Real-time PCR and Western blotting was applied to detect the mRNA and protein expression levels of PD-L1 and hypoxia-inducible factor-1α (HIF-1α) in HepG2 cells treated with 0, 2.5, 5, 10 μmol/L sorafenib, respectively.HepG2 cells were transfected with pEGFP-N1-HIF-1α (pEGFP-N1-HIF-1α group), shRNA-HIF-1α (shRNA-HIF-1α group) and the control plasmids including pEGFP-N1 and shRNA-control (pEGFP-N1 group, shRNA-control group), and then the mRNA and protein expression levels of HIF-1α and PD-L1 were detected.Results The mRNA and protein expression levels of PD-L1 and HIF-1α in HepG2 cells were down-regulated after sorafenib intervention in a dose-dependent manner (both P<0.05).Compared with the pEGFP-N1 group, the mRNA and protein expression levels of PD-L1 and HIF-1α increased in the pEGFP-N1-HIF-1α group (both P<0.01).Meanwhile, compared with the shRNA-control group, the mRNA and protein expression levels of PD-L1 and HIF-1α significantly decreased in the shRNA-HIF-1α group (both P<0.01).Conclusions Sorafenib can inhibit the expression of PD-L1 in liver cancer cells through inhibiting HIF-1α signaling pathway.关键词
肝肿瘤/索拉非尼/程序性死亡分子配体1/缺氧诱导因子1αKey words
liver neoplasms/sorafenib/programmed cell death ligand 1/hypoxia-inducible factor-1α分类
医药卫生引用本文复制引用
刘林森,龙文汀,张悦,刘树旺,刘利平..索拉非尼对肝癌细胞中PD-L1表达的影响及机制[J].山东医药,2017,57(29):5-7,3.基金项目
国家自然科学基金青年基金项目(81402041) （81402041）
深圳市科技创新项目(JCYJ20160422170206664,JCYJ20150403101028198). （JCYJ20160422170206664,JCYJ20150403101028198）
