山东医药2017，Vol.57Issue(29)：12-15,4.DOI:10.3969/j.issn.1002-266X.2017.29.004
蛛网膜下腔内注射TSA对RTX诱导神经病理性疼痛的影响及机制
Effects of subarachnoid injection of TSA on RTX-induced neuropathic pain and its mechanism
摘要
Abstract
Objective To study the effects of subarachnoid injection of trichostatin A (TSA) on resiniferatoxin (RTX)-induced neuropathic pain in rats and its mechanism.Methods Twenty-four male SD rats were randomly divided into four groups (n=6): Vehicle group, RTX group, RTX+DMSO group, and RTX+TSA group.The models of neuropathic pain were established in the remaining three groups except Vehicle group.Subarachnoid catheterization was performed in the RTX+DMSO group and RTX+TSA group one week prior to modeling.Before the establishment of the models, the rats were sacrificed with von Frey filaments to detect the basal value of the pain, and the mechanical pain threshold was measured on the 1st, 3rd, 5th, and 7th days after modeling.Vehicle group was treated with a single peritoneal RTX solvent 1.5 mL;rats in the RTX group received intraperitoneal injection of RTX (210 μg/kg);from 60 min before RTX injection, 5% DMSO (10 μL) was injected into rats of the RTX+DMSO group intrathecally once a day for 7 days;from 60 min before RTX injection, TSA (0.5 μg/kg) with solvent of 5% DMSO was injected into rats of the RTX+TSA group intrathecally once a day.The expression of BDNF mRNA in the spinal cord (SC) and dorsal root ganglion (DRG) was measured by qRT-PCR at 2 h after last time of TSA injection.Results Compared with the Vehicle group, the RTX group, RTX+DMSO group, and RTX+TSA group displayed a significantly decrease in mechanical allodynia on the 3th, 5th, and 7th days (P<0.05).Compared with the RTX group and RTX+DMSO group, the RTX+TSA group had significantly higher mechanical pain threshold on 5th and 7th days (all P<0.05).There was no statistically significant difference between the RTX group and RTX+DMSO group (P>0.05).Compared with the Vehicle group, the expression of BDNF mRNA was down-regulated in the spinal cord and DRG in the RTX group and RTX+DMSO group (P<0.05).Compared with the RTX group and RTX+DMSO group, the expression of BDNF mRNA in the spinal cord was up-regulated in the RTX+TSA group (P<0.05).There was no statistically significant difference between the RTX+DMSO group and RTX group (P>0.05).Conclusion The subarachnoid injection of TSA can relieve RTX-induced neuropathic pain by up-regulating the expression of brain-derived neurotrophic factor (BDNF).关键词
神经病理性疼痛/曲古菌素A/脑源性神经营养因子/树酯毒素/大鼠Key words
neuropathic pain/trichostatin A/brain-derived neurotrophic factor/resiniferatoxin/rats分类
医药卫生引用本文复制引用
陈文佳,孟晓文,王丽娜,詹英,嵇富海,杨建平..蛛网膜下腔内注射TSA对RTX诱导神经病理性疼痛的影响及机制[J].山东医药,2017,57(29):12-15,4.基金项目
国家自然科学基金资助项目(81471136) （81471136）
江苏省青年医学人才基金资助项目(QNRC2016740). （QNRC2016740）
