山东医药2017，Vol.57Issue(29)：23-25,3.DOI:10.3969/j.issn.1002-266X.2017.29.007
E1A基因对三阴乳腺癌细胞侵袭迁移的影响及机制
Effects of E1A gene on migration and invasion of triple-negative breast cancer cells
摘要
Abstract
Objective To investigate the effects of E1A gene on the migration and invasion of triple-negative breast cancer (TNBC) cells and is mechanism.Methods The eukaryotic expressive vector containing E1A gene (pcDNA3.0-E1A) (E1A stable clone group) and pcDNA3.0 empty plasmid (empty-plasmid clone group) were separately transfected into MDA-MB-231 cells by the lipofectamine, and stable E1A positive clones were selected by G418.Meanwhile, the MDA-MB-231 cells without any transfection were taken as the blank control group.Then Western blotting was utilized to identify the stable clones.The migration and invasion abilities of cells in all groups were analyzed by Scratch test and Transwell assay, respectively.Besides, the protein level of HSPA5 in all groups was detected by Westernblotting.Results MDA-MB-231 cell clones stably transfected with pcDNA3.0-E1A plasmid (E1A clones) were successfully established by the G418.The cell migration and invasion abilities of E1A clones in the E1A stable clone group were much lower as compared with those of the blank control group and the empty-plasmid clone group (P<0.05), accompanied by the down-regulated expression of HSPA5 protein (all P<0.05).Conclusion E1A gene can significantly inhibit the cell invasion and migration of TNBC cells in vitro by E1A promoting the protein degradation of HSPA5.关键词
三阴乳腺癌/E1A/细胞侵袭/细胞迁移Key words
triple-negative breast cancer/E1A/cell invasion/cell migration分类
医药卫生引用本文复制引用
邰亦成,方琦,谈珂岚,黄贵,张凌焱..E1A基因对三阴乳腺癌细胞侵袭迁移的影响及机制[J].山东医药,2017,57(29):23-25,3.基金项目
江苏省科学技术厅科技成果(20150014) （20150014）
常州科技计划项目 (CJ20140028). （CJ20140028）
