山东医药2017，Vol.57Issue(30)：5-9,5.DOI:10.3969/j.issn.1002-266X.2017.30.002
银杏叶提取物对慢性间歇性低氧大鼠胰岛β细胞氧化应激的影响及机制探讨
Effects of ginkgo biloba extracts on oxidative stress of islet β-cells in chronic intermittent hypoxic rats
摘要
Abstract
Objective To investigate the effects of ginkgo biloba extracts (EGB) on oxidative stress of islet β-cells in rats with chronic intermittent hypoxia (CIH) and its mechanism.Methods Ten SD rats were placed in hypoxia chamber to simulate intermittent hypoxia to prepare CIH models, and the other 10 rats were fed under normoxic conditions.At the end of 12 weeks, the primary islet β-cells from CIH model rats and normoxic rats were extracted.The primary islet cells of the normoxic rats were used as the normoxic control group;the primary islet cells of CIH model rats were divided into the CIH model group, anti-Nrf2 antibody group, Nrf2 activator group, low-dose EGB group, medium-dose EGB group, and high-dose EGB group.Rats in the oxygen control group and CIH model group were cultured in DMEM medium;Nrf2 antibody was added to the Nrf2 antibody group;Nrf2 activator sulforaphane with final concentration of 20 μg/mL was added to the Nrf2 activator group;5, 10, and 20 mg/mL EGB were added to the low-dose EGB group, medium-dose EGB group, and high-dose EGB group, respectively.The cells were taken from rats in each group after 24-hour intervention.The content of reactive oxygen species (ROS), the content of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in the supernatant was measured.The expression levels of Nrf2-ARE signal pathway related protein Nrf2 and downstream antioxidant enzyme-target protein HO-1, NQO1 and γ-GCS were detected by Western blotting.Results Compared with the normoxic control group, MDA and ROS increased and GSH-Px decreased in the CIH model group (all P<0.01).Compared with the CIH model group, MDA and ROS increased and GSH-Px decreased in anti-Nrf2 antibody group (P<0.01 or P<0.05).In the Nrf2 activator group, medium-dose EGB group, and high-dose EGB group, MDA and ROS were reduced, and GSH-Px increased (P<0.01 or P<0.05).MDA and ROS were lower and GSH-Px was higher in the high-dose EGB group than in the low-dose EGB group (P<0.01 or P<0.05).Compared with the normoxic control group, the level of Nrf2 increased, and HO-1, NQO1 and γ-GCS decreased in the CIH model group (all P<0.01).Compared with the CIH model group, HO-1 and NQO1 decreased in the anti-Nrf2 antibody group (both P<0.01).In the Nrf2 activator group, medium-dose EGB group, and high-dose EGB group, Nrf2, HO-1, NQO1, and γ-GCS increased (P<0.01 or P<0.05).The levels of Nrf2, HO-1, NQO1, and γ-GCS in the high-dose EGB group were higher than those of the medium-dose EGB group (P<0.01 or P<0.05).Conclusion EGB can reduce the level of oxidative stress in islet cells caused by CIH, and its mechanism may be related to the activation of Nrf2-ARE signaling pathway.关键词
慢性间歇性低氧/胰岛β细胞/银杏叶提取物/氧化应激/核因子2相关因子/阻塞性睡眠呼吸暂停综合征Key words
chronic intermittent hypoxia/islet β-cells/oxidative stress/ginkgo biloba extract/nuclear factor 2 related factor/obstructive sleep apnea syndrome分类
医药卫生引用本文复制引用
李无双,周燕,周璇..银杏叶提取物对慢性间歇性低氧大鼠胰岛β细胞氧化应激的影响及机制探讨[J].山东医药,2017,57(30):5-9,5.基金项目
国家自然科学基金资助项目(81460019). （81460019）
