山东医药2017，Vol.57Issue(30)：18-21,4.DOI:10.3969/j.issn.1002-266X.2017.30.005
合并与不合并2型糖尿病的下肢动脉硬化闭塞症患者血清差异性蛋白组学分析
Proteomics analysis on serum differential proteins between lower limb arteriosclerosis obliterans patients with and without type 2 diabetes mellitus
摘要
Abstract
Objective To identify the differentially expressed proteins between lower limb arteriosclerosis obliterans patients with type 2 diabetes mellitus (DLASO) and patients without type 2 diabetes mellitus (LASO) by the proteomic research.Methods Serum samples were separately collected from DLASO patients and LASO patients (15 cased in each group).Isobaric tags for relative and absolute quantitation (iTRAQ)-tagging combined with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) analysis were used to identify the differentially expressed proteins between DLASO patients and LASO patients.The differentially expressed proteins were analyzed for biological processes, molecular effects and cell composition by using PANTHER database.Results Compared with LASO patients, a total of 27 differentially expressed proteins were identified in DLASO patients, 8 proteins were significantly up-regulated while 19 proteins were significantly down-regulated.Biological process analysis showed that the differential protein was mainly involved in cell reaction (48.1%), metabolic process (48.1%), and bioadhesion (29.6%).The results of molecular analysis showed that the differential protein was mainly involved in cell binding (33.3%), catalytic activity (29.6%), and structural molecule activity (18.5%).Cell composition analysis showed that the differential proteins were mainly located in the cell region (14.8%), organelles, (14.8%) and extracellular regions (14.8%).Conclusion Totally 27 differentially expressed proteins between DLASO and LASO patients are identified by proteomic research, which may be used as potential biological targets for the prevention and treatment of DLASO.关键词
2型糖尿病/下肢动脉硬化闭塞症/蛋白组学/同位素标记/二维液相色谱串联质谱/差异性蛋白Key words
type 2 diabetes mellitus/lower limb arteriosclerosis obliterans/proteomics/isotope labelling/two-dimensional liquid chromatography-tandem mass spectrometry/differentially expressed proteins分类
医药卫生引用本文复制引用
杨晓虎,林裕辉,徐欣,范隆华..合并与不合并2型糖尿病的下肢动脉硬化闭塞症患者血清差异性蛋白组学分析[J].山东医药,2017,57(30):18-21,4.基金项目
上海市科学技术发展基金资助项目(11ZR1406900). （11ZR1406900）
