摘要
Abstract
Objective To investigate the effects of ultrasound-targeted microbubble destruction (UTMD) combined with liposome-mediated silencing specific nuclear matrix protein 1 gene (pSilencer3.1-SATB1) on transfection of human prostate cancer DU145 cells.Methods The DU145 cells were cultured in vitro and divided into 6 groups: the control group, microbubble group, ultrasound group, ultrasound+microbubble group, liposome group, and ultrasound+microbubble+liposome group.The control group did not do any treatment, the microbubble group was added with the appropriate amount of microbubbles, the ultrasound group only received ultrasonic irradiation, the ultrasound+microbubble group was added with microbubbles, followed by ultrasonic irradiation, the liposome group was added with liposome, and the ultrasound+microbubble+liposome group was added with microbubbles and liposomes, followed by ultrasonic irradiation.After 24-hour treatment, the expression of enhanced green fluorescent protein (EGFP) was observed by flow cytometry, the percentage of EGFP fluorescent cells was detected, and the transfection efficiency was evaluated.Results Fluorescence microscopy showed that the expression of green fluorescent protein in the ultrasound+microbubble+liposome group was numerous, which was significantly higher than that of the other groups.The transfection efficiencies of the control group, microbubble group, ultrasound group, ultrasound+microbubble group, liposome group, and ultrasound+microbubble+liposome group were 0.18%±0.09%, 0.25%±0.11%, 1.14%±0.13%, 2.96%±0.16%, 3.99%±0.12%, and 7.16%±0.17%, respectively, and the transfection efficiency of the ultrasound+microbubble+liposome group was higher than that of the other groups (all P<0.05).Conclusion UTMD can enhance the transfection efficiency of liposome-mediated plasmid pSilencer3.1-SATB1 to DUl45 cells, and improve the transfection efficiency, so it provides a new way for the treatment of prostate cancer.关键词
超声靶向微泡破坏/基因转染/脂质体/质粒/特异性核基质蛋白1基因/前列腺癌Key words
ultrasound-targeted microbubble destruction/gene transfection/liposome/plasmid/specific nuclear matrix protein 1 gene/prostate carcinoma分类
医药卫生
