山东医药2017，Vol.57Issue(31)：5-8,4.DOI:10.3969/j.issn.1002-266X.2017.31.002
粒细胞-巨噬细胞集落刺激因子转染对白血病细胞K562形态及增殖的影响
Effects of granulocyte-macrophage colony-stimulating factor transfection on morphology and proliferation of leukemia cells K562
摘要
Abstract
Objective To observe the effects of exogenous granulocyte-macrophage colony-stimulating factor (GM-CSF) and recombinant PcDNA3.1-GM-CSF on the morphology and proliferation of myeloid leukemia cells K562.Methods K562 cells were randomly divided into four groups.Recombinant PcDNA3.1-GM-CSF, exogenous GM-CSF, and PcDNA3.1 null plasmids were transfected into K562 cells as the recombinant plasmid group, the exogenous group and the empty plasmid group, respectively, and the non-transfected plasmid was set as the control group.The morphological changes of cells were observed under the optical microscope by using high power lens and oil lens, respectively, and the cell proliferation was measured by MTT.Results Morphological changes seen under high power lens: the control group, the empty plasmid group and exogenous group were similar in morphology, cells were stained dark blue, whereas no clear boundary was seen between the cytoplasm and nucleus;compared with these three groups, the cells of the recombinant plasmid group showed a significant difference, the cytoplasm was stained light blue, and the nuclei were pale pink along with a clear bounds between them.Kidney or horseshoe shaped nuclei could be seen in some of the cells.Morphological changes seen under oil lens: the control group, the exogenous group, and empty plasmid group were similar, the cells were stained dark blue, and granules deposition wasn''''t seen in the cells;as to the recombinant plasmid group, some cells were stained dark blue, while some were pale pink in which dotted formazan particles were seen.Results of cell proliferation assay: Compared with the other two groups, the inhibition rate of cell proliferation in the recombinant plasmid group showed an increase on the 3th , 5th, 7th and 9th days (P<0.05).Compared with the empty plasmid group, the inhibition rate in the exogeneous group displayed an increase on the 3th, 5th and 7th days (P<0.05).Conclusion Both recombinant PcDNA3.1-GM-CSF and exogenous GM-CSF could induce morphological change and lead to proliferation inhibition in K562 cells, the effects increase along with time, and the inhibitory effect of recombinant PcDNA3.1-GM-CSF is stronger than that of the exogenous GM-CSF.关键词
粒细胞-巨噬细胞集落刺激因子/白血病细胞/细胞形态/细胞增殖Key words
granulocyte-macrophage colony-stimulating factor/leukemic cells/cell morphology/cell proliferation分类
医药卫生引用本文复制引用
陈蓉,常晋霞,李雪璐,彭丽娟,朱红..粒细胞-巨噬细胞集落刺激因子转染对白血病细胞K562形态及增殖的影响[J].山东医药,2017,57(31):5-8,4.基金项目
四川省教育厅自然科学基金资助项目(12ZB214). （12ZB214）
