山东医药2017，Vol.57Issue(31)：9-12,4.DOI:10.3969/j.issn.1002-266X.2017.31.003
结直肠癌患者粪便p16INK4a、MGMT、RASSF1A基因甲基化状态观察
Observation of p16INK4a, MGMT, and RASSF1A gene methylation in stool of patients with colorectal cancer
摘要
Abstract
Objective To observe the gene promoter methylation of p16INK4a, MGMT, and RASSF1A in stool of patients with colorectal cancer (CRC).Methods Fifty healthy examined people (control group), 50 patients with benign colorectal disease (benign colorectal disease group) and 50 CRC patients (CRC group) were enrolled in the study.The p16INK4a, MGMT and RASSF1A promoter methylation detection rates in stool were detected by metilylation specific PCR (MSP).The correlation between p16INK4a, MGMT and RASSF1A promoter methylation levels and clinical parameters were analyzed by using statistic method.The sensitivity and specificity of p16INK4a, MGMT, and RASSF1A alone and in combination for the diagnosis of CRC were compared by using Bay''''s equation on the basis of pathological diagnosis.Results The detection rates for p16INK4a methylation were 74%, 28%, and 14% in the CRC group, benign colorectal disease group, and control group, 56%, 24%, and 12% for MGMT gene, and 72%, 20%, and 10% for RASSF1A gene, respectively.Statistically significant difference was found between the control group, benign colorectal disease group, and CRC group (all P<0.05).Additionally, correlation analysis showed that both p16INK4a and RASSF1A gene methylation was significantly associated with differentiated degree of CRC, TNM stage, and lymph node metastasis, while MGMT was correlated with lymph node metastasis (all P<0.05).The diagnostic sensitivity and specificity for combination of p16INK4a, MGMT, and RASSF1A was 95.8% and 83.4%, respectively.The ROC area under curve was 0.816 (95%CI: 0.739%-0.894%).Conclusions The methylation levels of p16INK4a, MGMT and RASSF1A in stool from CRC patients are significantly higher than those of patients with benign colorectal disease and healthy people.The combined detection of these three genes in stool is useful for early diagnosis of CRC and prediction of biological function of CRC.关键词
结直肠癌/p16INK4a基因/MGMT基因/RASSF1A基因/基因甲基化Key words
colorectal carcinoma/p16INK4a gene/MGMT gene/RASSF1A gene/gene methylation分类
医药卫生引用本文复制引用
何樱,黄维甄,欧阳考滨,袁霞..结直肠癌患者粪便p16INK4a、MGMT、RASSF1A基因甲基化状态观察[J].山东医药,2017,57(31):9-12,4.基金项目
广东省惠州市民政局资助项目(20151116344). （20151116344）
