实用医学杂志2017，Vol.33Issue(16)：2620-2625,6.DOI:10.3969/j.issn.1006-5725.2017.16.004
RYBP介导白血病细胞凋亡的靶基因预测
Prediction of the target genes mediated by RYBP in the leukemic cell apoptosis
摘要
Abstract
Objective To investigate the target genes in RYBP-mediated leukemia cell apoptosis by high-flux sequencing. Methods The HL-60 cell line with knockdown of RYBP was set up. MRNA and microRNA sequencing was conducted by the next-generation sequencing technology. Result MRNAs and miRNAs were dys-regulated in HL-60 cell line with knockdown of RYBP. The results of KEGG analysis indicated that the down-regu-lated genes were associated with cancer transcription regulation and amino acid metabolism. SPI1 and miR-214-3p were shown downregulated after knockdown of RYBP. Conclusion The target genes in RYBP-mediated leukemia cell apoptosis include BBC3,BAI1,SESN2 ,CCNG2,JAK3,STAT4,SPI1,BCL6,CD11b and hsa-miR-214-3p.关键词
RYBP/白血病/二代测序/靶基因Key words
RYBP/second-generation sequencing/target gene引用本文复制引用
李秋兰,王顺清,张玉平,陈晓燕,谢健晋,吴亮亮..RYBP介导白血病细胞凋亡的靶基因预测[J].实用医学杂志,2017,33(16):2620-2625,6.基金项目
广东省自然科学基金(编号:2014A030313676) （编号:2014A030313676）
中华医学会临床医学科研专项资金(编号:16010050621) （编号:16010050621）
广州市科技计划项目(编号:201508020254) （编号:201508020254）
