实用医学杂志2017，Vol.33Issue(16)：2642-2645,4.DOI:10.3969/j.issn.1006-5725.2017.16.009
普罗布考减轻氧糖剥夺/复氧小鼠睾丸支持细胞损伤
Probucol alleviating mouse Sertoli cell injury induced by oxygen-glucose deprivation/reoxygenation
李莉 1龚淼 1王慧娟 1赵秀军 1王娜2
作者信息
- 1. 050017 石家庄市,河北医科大学组织胚胎学教研室
- 2. 050200 河北中医学院生理教研室
- 折叠
摘要
Abstract
Objective To investigate the effect of probucol on Sertoli cell injury induced by oxygen-glucose deprivation/reoxygenation(OGD/R)and mechanism. Methods Primary Sertoli cells treated with oxygen glucose deprivation for 2 h and reoxygenation for 3 h were modeled. We incubated Sertoli cells with probucol in a concentration of 5,10,and 20 μmol/L from reoxygenation to the end. The cell viability was evaluated using Cell Proliferation Assay kit. ROS production was observed by flow cytometry. The levels of MDA ,iron and GSH or the activity of glutathione-dependent peroxidase were measured by Assay Kit. Results The ferroptosis inhibitors pre-vented cell death(P < 0.01). The cell death rate was decreased by probucol in time- and dose-dependent manner (P < 0.01). Meanwhile, probucol caused increase of ROS,MDA,GSH content and GPXs activity(P < 0.05). Conclusion Probucol protects Sertoli cells from OGD/R damage via inhibiting lipid peroxidation-mediated ferroptosis.关键词
Ferroptosis/支持细胞/FPN/GPXs/脂质过氧化Key words
ferroptosis/sertoli cell/FPN/GPXs/lipid peroxidation引用本文复制引用
李莉,龚淼,王慧娟,赵秀军,王娜..普罗布考减轻氧糖剥夺/复氧小鼠睾丸支持细胞损伤[J].实用医学杂志,2017,33(16):2642-2645,4.
