实用医学杂志2017，Vol.33Issue(16)：2666-2669,4.DOI:10.3969/j.issn.1006-5725.2017.16.015
血清外泌体miR-21在肺癌中的表达水平及其诊断效能
Expression level and diagnosis performance of serum exosomal miR-21 in lung cancer
摘要
Abstract
Objective To observe the expression level and diagnosis performance of serum exosomal miR-21 in lung cancer. Method Sera of 94 lung cancer patients,29 pulmonary tuberculosis patients,and 26 healthy controls from Nan Fang Hospital were collected. Exosomes in serum were purified and characterized ,and miRNA was abstracted from exosomes. Levels of exosomal miR-21 in three groups were compared with relative quantitative RT-PCR. Diagnosis performance of exosomal miR-21 was evaluated by ROC curve. Results Purified serum exosome sample possesses typical exosome features. There were significant difference among exosomal miR-21 levels from lung cancer ,tuberculosis ,and health control group. Exosomal miR-21 obviously increased in lung cancer patients,and decreased in tuberculosis patients. Exosomal miR-21 were increased in early and late stage lung cancer patients. The diagnostic performance of exosomal miR-21 was evaluated by ROC curve. Its area under the curve was 0.702 and specificity was 0.923. Conclusions Serum exosomal miR-21 was increased in many kinds of lung can-cer patients. It possesses excellent diagnosis performance ,which may be developed as new biomarkers to assist lung diagnosis.关键词
外泌体/血清/肺癌/诊断标志物/miR-21Key words
exosomes/serum/lung cancer/diagnostic biomarker/miR-21引用本文复制引用
颜晓慧,安泰学,覃思华,徐咏,黄依瑶,张涵..血清外泌体miR-21在肺癌中的表达水平及其诊断效能[J].实用医学杂志,2017,33(16):2666-2669,4.基金项目
广东省自然科学基金(编号:2015A030313293) （编号:2015A030313293）
广东省科技计划项目(编号:2013B021800314) （编号:2013B021800314）
广州市科技计划项目(编号:1563000220) （编号:1563000220）
