生殖医学杂志2017，Vol.26Issue(8)：820-826,7.DOI:10.3969/j.issn.1004-3845.2017.08.015
生物信息学方法研究高脂饮食诱导肥胖对雄性SD大鼠的影响
Effects of high-fat diet induced obesity on SD male rat by using bioinformatics analysis
贾艳飞 1郭颖 2张蔚 2张开舒 1傅龙龙 2安琪 1童越 2谷翊群1
作者信息
- 1. 北京协和医学院研究生院,北京 100730
- 2. 国家卫生计生委科学技术研究所,北京 100081
- 折叠
摘要
Abstract
Objective: To investigate the effect of high-fat diet induced obesity on physiological metabolism and fertility in SD male rats.Methods: By using GEO gene chip common database in NCBI for chip data search,finally the chip data(GSE8700) was selected as the analysis object.The differentially expressed genes(DEGs) were identified by using R software in the bioconductor package and the Limma package.GO enrichment and KEGG pathway analysis were performed using DAVID online tool.Protein-protein interaction(PPI) network of the DEGs was constructed by String database.Results: A total of 1 014 genes were identified after analyzing GSE8700.Among them,544 genes were up-regulated and 470 genes were down-regulated.GO analysis results showed that up-regulated DEGs were significantly enriched in biological processes(BP),including oxidation-reduction process,axon formation and response to glucocorticoid.The down-regulated DEGs were significantly enriched in biological processes,including female pregnancy,response to steroid hormone and triglyceride metabolic process,etc.Go cell component(CC) analysis only displayed in down-regulated DEGs,including extracellular space,cytoplasm and blood microparticle.For molecular function(MF),the DEGs were enriched in serine-type endopeptidase activity,fatty acid binding and phospholipid binding;the up-regulated genes were not enriched in any KEGG pathway,and the down-regulated DEGs were enriched in PPAR signaling pathway,fat digestion and absorption,pancreatic secretion.The top 10 hub genes included heat shock protein 90 alpha family class B member 1(Hsp90ab1),calcium-sensing receptor(Casr),and chemokine(C-C motif) ligand 9(Ccl9),etc.Conclusions: High-fat diet induced obesity can lead to the lipid metabolism disorders in male rats.Obesity may affect the reproductive function of male rat.Its mechanism might be related to the metabolic abnormality of steroids hormone and peptide hormone.关键词
生物信息学/肥胖/生殖/差异基因Key words
Bioinformatics analysis/Obesity/Reproduction/Differentially expressed gene引用本文复制引用
贾艳飞,郭颖,张蔚,张开舒,傅龙龙,安琪,童越,谷翊群..生物信息学方法研究高脂饮食诱导肥胖对雄性SD大鼠的影响[J].生殖医学杂志,2017,26(8):820-826,7.
