天津中医药大学学报2017，Vol.36Issue(4)：282-285,4.DOI:10.11656/j.issn.1673-9043.2017.04.12
ox-LDL对内皮细胞CD40信号表达的影响及黄芪甲苷的抗炎作用
Effect of the expression of CD40 signal in endothelial cells with ox-LDL and anti-inflammatory effect of Astragaloside IV
摘要
Abstract
[Objective] Effect of the expression of CD40 signaling in endothelial cells (ECV-304) with modified low density lipoprotein (ox-LDL) and anti-inflammatory effects of Asragaloside IV. [Methods] ECV-304 with 100 mg/L ox-LDL for 24 h, then measured the expression of CD40 mRNA and protein by Real-time PCR and Western blot. The content of TNF, sVCAM-1, IL-6 and IL-8 in cell supernatant were determined by ELISA assay. With anti-CD40 antibody was added to ECV-304, and so did the same of TNF-α, sVCAM-1, IL-6 and IL-8 in cell supernatant. ECV-304 with 20 g/mL Astragaloside IV preincubation for 4 h, and then with 100 mg/L ox-LDL for another 24 h, checking the CD40 mRNA and protein and the content of TNF-α, sVCAM-1, IL-6 and IL-8 in cell supernatant. [Results] The expression of CD40 mRNA protein were up-regulated with Ox-LDL in ECV-304, and promoted cell secretion of TNF-α, sVCAM-1, IL-6 and IL-8.However, the protein of CD40 were inhibited, and TNF-α, sVCAM-1, IL-6 and IL-8 were all down-regulated. Astragaloside IV could down regulate the expression of CD40 mRNA and protein, and inhibit the secretion of TNF-α, sVCAM-1, IL-6 and IL-8. [Conclusion] Astragaloside IV could inhibit the expression of ox-LDL induced inflammatory mediators in endothelial cells by inhibiting CD40 signaling, which is a potential mechanism of anti-atherosclerosis.关键词
内皮细胞/氧化修饰的低密度脂蛋白/CD40/抗炎症/动脉粥样硬化Key words
endothelial cells/ox-LDL/CD40/anti-Inflammatory/atherosclerosis分类
医药卫生引用本文复制引用
魏冰,姜希娟,卢斌,孙英新,李虎虎,张同,李悠悠,郭茂娟..ox-LDL对内皮细胞CD40信号表达的影响及黄芪甲苷的抗炎作用[J].天津中医药大学学报,2017,36(4):282-285,4.基金项目
国家自然科学基金项目(81573733). （81573733）
