天津中医药大学学报2017，Vol.36Issue(4)：291-294,4.DOI:10.11656/j.issn.1673-9043.2017.04.14
人子宫内膜腺上皮细胞的体外分离培养及纯化
Isolation, culture and purification of human endometrial glandular epithelial cells in vitro
摘要
Abstract
[Objective] Improvement the isolation, culture and purification technology of human endometrial glandular epithelial cells to obtain lots of the economic cells with good activity and high purity , and provide reliable model in vitro to study of endometriosis. [Methods] Repeated digestion of composite enzyme, single filtration of screen mesh with 220μm in diameter, low speed centrifugal and differences in time of adherent cells. [Results] The human endometrial glandular epithelial cells were successfully cultured in 18 of 19 endometrial tissues, which were obtained from the method of diagnostic curettage. The putity of human endometrial glandular epithelial cells was up to 94.2%. [Conclusion] By the methods of repeated digestion of composite enzyme, single filtration of screen mesh with 220μm in diameter, low speed centrifugal and differences in time of adherent cells, we can obtain lots of the economic cells with good activity and high purity.关键词
子宫内膜异位症/腺上皮细胞/细胞培养Key words
endometriosis/endometrial glandular epithelial cells/cells culture分类
医药卫生引用本文复制引用
王月玲,李沛霖,李洁,王建玲,华丹丹,王倩男,王璀..人子宫内膜腺上皮细胞的体外分离培养及纯化[J].天津中医药大学学报,2017,36(4):291-294,4.基金项目
国家自然科学基金项目(81072837). （81072837）
