外科理论与实践2017，Vol.22Issue(4)：327-330,4.DOI:10.16139/j.1007-9610.2017.04.012
T细胞免疫功能重建影响裸鼠胰腺癌淋巴转移的实验研究
Effect of immunological function reconstruction of T lymphocyte on lymphatic metastasis of pancreatic cancer in nude mice
阎九亮 1李宁 1杜卫东 1黄捷 1叶志鹏 1傅永清1
作者信息
- 1. 浙江中医药大学附属第一医院肝胆外科,浙江 杭州 310006
- 折叠
摘要
Abstract
Objective To provide T lymphocyte model of immunological function reconstruction and to study the as-sociation of immunity with lymphatic metastasis of pancreatic cancer. Methods Both the nude mice model of pancreatic cancer with lymphatic metastasis and T lymphocyte model of immunological functions reconstruction were established. Nude mice were divided into 2 groups. The immunological function was reconstructed in the nude mice as R1 group and the nude mice without immunological functions reconstruction were as R2 group. The time of tumor formation in the nude mice were compared between 2 groups. Lymph nodes metastases in nude mice were examined histologically. CD3+CD4+and CD3+CD8+ in the peripheral blood and spleen of nude mice were detected by flow cytometry. Results The time of tumor formation was (8.5±0.6) days in R1 group and (6.4±0.8) days in R2 group, and the difference was statistically signi-ficant (P<0.05). Lymphatic metastases of pancreatic cancer were inhibited at some extent in the nude mice in R1 group. The rate of metastases to common iliac artery lymph nodes in R1 group was higher than that in R2 group. The ratio of CD3+CD4+and CD3+CD8+in the peripheral blood and spleen of the nude mice in R1 group was higher than that in R2 group significantly. Conclusions The model of T lymphocyte in nude mice with immunological functions reconstruction in this study could provide a good immunological platform for the study on immunological functions associated with lymphatic metastasis of pancreatic cancer.关键词
T细胞/胰腺癌/免疫功能/淋巴转移Key words
T lymphocytes/Pancreatic cancer/Immunological functions/Lymphatic metastasis分类
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阎九亮,李宁,杜卫东,黄捷,叶志鹏,傅永清..T细胞免疫功能重建影响裸鼠胰腺癌淋巴转移的实验研究[J].外科理论与实践,2017,22(4):327-330,4.
