新医学2017，Vol.48Issue(8)：550-554,5.DOI:10.3969/j.issn.0253-9802.2017.08.008
胰岛素信号转导通路相关基因多态性与阿尔茨海默病的关系
Relationship between insulin signal transduction pathway-associated gene polymorphism and Alzheimer disease
摘要
Abstract
Objective To investigate the relationship between insulin signal transduction pathway-associated gene phosphatidylinositol 3 kinase (PI3K) polymorphism and Alzheimer disease (AD).Methods In this study, 231 AD patients(AD group) and 231 healthy subjects (healthy control group) were recruited.Peripheral venous blood sampling was collected for DNA detection.The gene polymorphism of PI3K was detected by PCR and single nucleotide polymorphism sequencing.The serum level of PI3K protein was measured by enzyme linked immunosorbent assay (ELISA).The PI3K genotype, allele distribution and PI3K protein level were statistically compared between two groups.The relationship between PI3K polymorphism rs3730087 and expression level of PI3K protein was analyzed.Results The genotype and allele of PI3K polymorphism reference IDPIK3R1 rs3730087 and the PI3K protein level significantly differed between the AD and healthy control groups (P<0.05).The expression levels of PI3K protein with varying gene polymorphisms significantly differed between TT and TC (P=0.004), TT and CC in the AD group (P=0.016), TT and TC(P=0.001), TT and CC(P=0.022)in the healthy control group.Conclusions The insulin signal transduction pathway-associated gene PI3K polymorphism exerts certain effect upon AD.PIK3R1 rs3730087 (T>C) increases the risk of AD.关键词
基因多态性/胰岛素信号/阿尔兹海默病/转导通路Key words
Gene polymorphism/Insulin signal/Alzheimer disease/Transduction pathway引用本文复制引用
郑南,赵和丹,田华伟,王金龙,黄通,李小玲,周国强,刘盛元..胰岛素信号转导通路相关基因多态性与阿尔茨海默病的关系[J].新医学,2017,48(8):550-554,5.基金项目
广东省医学科研基金资助项目(A2015387) （A2015387）
