疑难病杂志2017，Vol.16Issue(9)：927-930,4.DOI:10.3969/j.issn.1671-6450.2017.09.016
实脾固肾化瘀方对阿霉素肾纤维化大鼠肾组织TGF-β1/Smads信号通路的影响
Effects of Shipigushenhuayufang on the TGF-β1/Smads signaling pathway in the kidney tissue of adriamycin induced renal fibrosis rats
摘要
Abstract
Objective To investigate the effect of Shipigushenhuayufang on transforming growth factor-β1 (TGF-β1) /Smads signaling pathway in renal tissue of adriamycin (ADR) rats with renal fibrosis.Methods The study was conducted at the Institute of nephropathy, affiliated Shuguang Hospital of Shanghai University of Traditional Chinese Medicine from Aug 2016 to December 2016.40 male SD rats were randomly divided into 4 groups (10 rats in each group): normal control group, model group, Shipigushenhuayufang group, and fosinopril sodium group.Except the normal control group, the rats in the other 3 groups were injected with ADR (4 mg/kg) into the renal fibrosis model.At the beginning of the third week of the model, the Shipigushenhuayufang group was treated with Shipigushenhuayufang (43 g/kg);the fosinopril sodium group was treated with fosinopril sodium solution (2 mg/kg);the model group and the normal control group were treated with normal saline.At the end of the experiment, 24 h urine volume, serum and kidney tissue were taken from all rats.The pathological changes of kidney and the changes of the expressions of TGF-β1, Smad2 and Smad7 in renal tissue were observed in each group.Results Masson staining showed that the normal control rats were normal in morphology and structure, and no abnormal changes were found in renal tubules,interstitial fibrosis and inflammatory cell infiltration.The collagen fibers in the kidney were weakly expressed, the collagen fibers were blue, and the muscle fibers and erythrocytes were red.The pathological damage appeared in the renal tissue of the rats in the model group, Shipigushenhuayufang group, fosinopril sodium group had pathological damage significantly reduced, and the Shipigushenhuayufang group rat improve the pathological kidney tissue more obvious than fosinopril sodium group.Immunohistochemical staining showed that, compared with normal control group, TGF-β1 expression was significantly increased in renal tissue of model group (P <0.01), the expression of Smad2 in renal tissue of model group increased significantly (P <0.01), compared with the model group, Shipigushenhuayufang group rat kidney TGF-β1 and the expression of Smad2 was down regulated (P <0.01), Smad7 expression up regulated (P <0.01).Compared with the fosinopril sodium group, the expression of TGF-β1 and Smad2 in the renal tissue of the Shipigushenhuayufang group was not statistically significant different (P >0.05), while the expression of Smad7 was significantly higher (P <0.05).Conclusion The Shipigushenhuayufang may regulate the expression of TGF-β1 and Smad2, and up regulate the expression of Smad7, and interfere with the TGF-β1/Smads signaling pathway, thereby improving the renal fibrosis and protecting the renal function in ADR nephropathy rats.关键词
实脾固肾化瘀方/阿霉素/肾纤维化/TGF-β1/Smad2/Smad7Key words
Shipigushenhuayufang/Adriamycin/Renal fibrosis/TGF-β1/Smad2/Smad7引用本文复制引用
王高强,米秀华,沈丽萍,张玮,李珺,倪家庆..实脾固肾化瘀方对阿霉素肾纤维化大鼠肾组织TGF-β1/Smads信号通路的影响[J].疑难病杂志,2017,16(9):927-930,4.基金项目
上海市卫生局中医药科研基金项目资助(2008J013A) （2008J013A）
上海市中医药发展三年行动计划中医药创新内涵建设项目资助(ZY3-CCCX-3-3016) （ZY3-CCCX-3-3016）
上海市"杏林新星"人才培养项目资助(ZY3-RCPY-2-2087) （ZY3-RCPY-2-2087）
