疑难病杂志2017，Vol.16Issue(9)：931-934,4.DOI:10.3969/j.issn.1671-6450.2017.09.017
橄榄苦苷对HK-2细胞缺糖缺氧损伤的保护作用
Protective effect of oleuropein on glucose deprivation hypoxia injury in HK-2 cells
摘要
Abstract
Objective To investigate the protective effect of oleuropein and its possible mechanism on the glucose deprivation hypoxia injury of HK-2 cells.Methods From January 2017 to April 2017, the experiment was conducted at the Central Laboratory of Renmin Hospital of Wuhan University.HK-2 cells were cultured in vitro, using sugar free serum free culture medium and three gas incubators to establish the model of oxygen glucose deprivation, the cells were divided into control group, hypoxia 24 h reoxygenation 3 h group (IR group) and 24 h of hypoxia reoxygenation for 3 h with different concentrations (10 μmol/L, 50 μmol/L, 100 μmol/L, 200 μmol/L) of oleuropein in intervention group.With the detected CCK-8 A450 value, compared with the normal group the survival rate calculated cells, the apoptosis cells were detected with Hoechst staining, Western Blot assay of B lymphocyte tumor 2 (B cell lymphoma 2, Bcl-2), Bcl-2 X protein (Bcl-2 associated X protein, Bax) expression of protein.Results With the increase of concentration of oleuropein can improve the survival rate of HK2 cells in OGD environment, 100 μmol/L group showed the highest survival rate, but the survival rate in 200 μmol/L group decreased;Hoechst staining showed that oleuropein could reduce apoptosis induced by OGD injury, lower apoptosis rate, the apoptosis rate of 100 μmol/L group was the lowest;Western blot results showed that oleuropein could increase the expression of Bcl-2 protein and decrease the expression of Bax.Conclusion It has a protective effect on glucose deprivation and hypoxia injury in HK-2 cells, and its mechanism may be related to its inhibition of apoptosis.关键词
橄榄苦苷/HK-2细胞/缺糖缺氧损伤/细胞凋亡Key words
Oleuropein/HK-2 cell/Oxygen glucose deprivation/Apoptosis引用本文复制引用
徐涛,秦聪,肖程程,郑海洲,张杰..橄榄苦苷对HK-2细胞缺糖缺氧损伤的保护作用[J].疑难病杂志,2017,16(9):931-934,4.基金项目
国家自然科学基金资助项目(81470923) （81470923）
