湖北医药学院学报2017，Vol.36Issue(3)：221-224,4.DOI:10.13819/j.issn.1006-9674.2017.03.006
武当地区马尾松松针中总黄酮的提取工艺优化及含量测定
Optimization of Extraction Process and Determination of Total Flavonoids in Pine Needles of Pinus massoniana in Wudang Area
摘要
Abstract
Objective To optimize the extraction technology of total flavonoids in Pinus massoniana needles in Wudang area and to establish a method for its determination.Methods The total flavonoids in Pinus massoniana needles obtained from Wudang Mountain were determined by UV spectrophotometry with rutin as the control.The effect of the ratio of solid to liquid,ultrasonic power and ultrasonic time on the content of total flavonoids was studied by orthogonal design.Results Lutin within the concentration range of 8.256～49.536 μg · mL-1 showed a good linear relationship.The optimal extraction conditions were as follows:solid to liquid ratio 1:50,ultrasound power 350 W,ultrasound time 45 rain,ultrasound temperature (70±3) ℃.Conclusion The optimal extraction technology is simple and rapid,and the content determination method is stable and reliable.It can be used for extracting and determinating total flavonoids in pine needles of Pinus massoniana in Wudang area.关键词
马尾松/松针/总黄酮/提取工艺/正交实验/含量测定/紫外分光光度法Key words
Pinus massoniana Lamb/Pine needles/Total flavonoids/Orthogonal test/Extraction technology/Content determination/UV spectrophotometry引用本文复制引用
皮婷婷,曹丹丹,郑芳,朱雪松,李聪..武当地区马尾松松针中总黄酮的提取工艺优化及含量测定[J].湖北医药学院学报,2017,36(3):221-224,4.基金项目
湖北省2011协同创新中心基金(2011JH-2014CXTT15) （2011JH-2014CXTT15）
十堰市科技局项目(14k73) （14k73）
2016年湖北医药学院药护学院创新创业项目(201613249010) （201613249010）
