针刺研究2017，Vol.42Issue(4)：308-314,7.DOI:10.13702/j.1000-0607.2017.04.005
电针"丰隆"穴对非酒精性脂肪肝大鼠肝组织固醇调节元件结合蛋白-1 c的影响
Effect of Electroacupunctrue Stimulation at"Fenglong"(ST 40) on Expression of SREBP-1 c in Non-alcoholic Fatty Liver Disease Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA) stimulation at "Fenglong"(ST 40) on expression of sterol regulatory element binding protein-1 c(SREBP-1 c) in non-alcoholic fatty liver disease(NAFLD) rats, so as to reveal its mechanism underlying improving NAFLD.Methods Male SD rats were randomly divided into normal diet group, high-fat diet group, ST 40 manual acupuncture (MA) group, ST 40 EA group and "Zusanli"(ST 36) EA group(n=12 in each group).Apart from the normal diet group, rats in other groups were fed with high-fat diet to replicate NAFLD model.ST 40 MA group was treated with filiform needling for 20 min, EA (4 Hz/20 Hz, 5 mA) was applied to bilateral acupoints of ST 40 and ST 36 respectively for 20 min in the ST 40 EA group and ST 36 EA group, once a day for 4 weeks.The pathological changes of hepatic tissue were observed by HE staining.The contents of serum free fatty acid (FFA), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected by colorimetry.The expression of SREBP-1 c mRNA and protein in the hepatic tissue were detected by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot, respectively.Results HE staining showed that hepatocytes disorganized with diffuse bullous fat vacuolar changes in rats of the high-fat diet group, and small vesicle steatosis appeared in the ST 40 MA group.The liver cells arranged orderly and the steatosis decreased in the ST 40 EA group and ST 36 EA group.The contents of FFA, ALT, AST in serum and the expression levels of SREBP-1 c mRNA and protein in the hepatic tissue were significantly higher in the high-fat diet group than in the normal diet group(P<0.01), while significantly lower in the MA and EA groups than in the high-fat diet group(P<0.01).Furthermore, the effects of treatment in the ST 40 EA group were better than those in the ST 40 MA group(P<0.01, P<0.05).The FFA contents in serum and the expression levels of SREBP-1 c mRNA and protein were decreased in the ST 40 EA group in comparison with the ST 36 EA group(P<0.05).Conclusion EA at ST 40 and ST 36 have a positive regulating effect on NAFLD rats, which may be related to down-regulating the expression of SREBP-1 c mRNA and protein in hepatic tissue, improving the endoplasmic reticulum stress and regulating lipid metabolic disorder, so as to relieve the inflammatory injury in hepatic tissue, and ST 40 works better.关键词
电针/非酒精性脂肪肝病/内质网应激/固醇调节元件结合蛋白-1cKey words
Electroacupuncture/Non-alcoholic fatty liver disease/Endoplasmic reticulum stress/Sterol regulatory element binding protein-1 c分类
医药卫生引用本文复制引用
余敏,李钢,唐成林,高睿琦,冯启廷,曹净..电针"丰隆"穴对非酒精性脂肪肝大鼠肝组织固醇调节元件结合蛋白-1 c的影响[J].针刺研究,2017,42(4):308-314,7.基金项目
重庆市卫生计生委资助项目(No.AZY201402078) （No.AZY201402078）
