针刺研究2017，Vol.42Issue(4)：315-320,6.DOI:10.13702/j.1000-0607.2017.04.006
电针对糖尿病胃轻瘫大鼠胃窦组织胰岛素生长因子1及其受体的影响
Effect of Electroacupuncture Intervention on Gastrointestinal Motility and Expression of Insulin-like Growth Factor 1 and Its Receptor Proteins in Gastric Antrum in Diabetic Gastroparesis Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) intervention of "Zusanli"(ST 36), etc.on gastrointestinal motility and levels of insulin-like growth factor 1 (IGF-1) and IGF-1 receptor (IGF-1 R) proteins in the gastric antrum in diabetic gastroparesis (DGP) rats, so as to study its mechanism underlying improvement of DGP.Methods Fifty SD rats, half male and half female, were randomly allocated to control, model, EA acupoint, EA non-acupoint and medication (metoclopramide) groups (n=10 rats/group).The DGP model was induced by 2% streptozotocin (STZ, i.p.i.,55 mg/kg) and high fat-sugar forage for 8 weeks.EA (10 Hz/50 Hz, 2 mA) was applied to "Zusanli"(ST 36), "Liangmen"(ST 21), "Sanyinjiao"(SP 6) and non-acupoints (about 5 mm lateral to ST 36, ST 21 and SP 6, respectively) for 20 min, one daily for 15 days, and 1.7% metocloppramide (1 mL/100 g) was given (gavage) to rats of the medication group, once daily for 15 days.After the treatment, the rats'' general conditions were scored, the blood glucose level was determined using a glucometer, and the gastrointestinal mobility was evaluated by measuring the gastric emptying rate (GER) and the intestinal propulsion rates (IPR) marked by oral-infused phenol red solution (50 mg/dL).The immunoactivity levels of IGF-1 and IGF-1 R of gastric antrum tissue were detected by enzyme-linked immunosorbent assay (ELISA).Results After modeling, the symptom integrative score, the blood glucose, IGF-1 and IGF-1 R levels were significantly increased (P<0.01), and the GER and IPR were notably decreased relevant to the control group (P<0.01).After EA stimulation of ST 36, ST 21 and SP 6, the symptom score, blood glucose, and IGF-1 and IGF-1 R levels of the gastric antrum were significantly reduced, while the GER and IPR were considerably increased (P<0.05,P<0.01).Except the GER and IPR were considerably increased in the medication group(P<0.05,P<0.01), no significant changes were found in the symptom integrative score, the blood glucose, IGF-1 and IGF-1 R levels in both EA non-acupoint and medication groups relevant to the model group (P>0.05),the GER and IPR in the EA non-acupoint group (P>0.05).Compared with the EA acupoint group, the symptom integrative score, IGF-1 and IGF-1 R levels were significantly increased in both EA non-acupoint and medication groups(P<0.05,P<0.01).Conclusion EA stimulation can improve gastrointestinal motility and lower blood glucose in DGP rats, which may be closely associated with its effectiveness in reducing IGF-1 and IGF-1 R immunoactivity levels in gastric antrum.关键词
电针/糖尿病胃轻瘫/胃动力/胰岛素样生长因子1/胰岛素样生长因子1受体/胃窦Key words
Electroacupuncture/Diabetic gastroparesis/Gastric motility/Insulin-like growth factor 1/Insulin-like growth factor 1 receptor/Gastric antrum分类
医药卫生引用本文复制引用
杨建文,彭艳,陈海交,张程程,刘薇薇,刘丽,刘密,林亚平..电针对糖尿病胃轻瘫大鼠胃窦组织胰岛素生长因子1及其受体的影响[J].针刺研究,2017,42(4):315-320,6.基金项目
国家重点基础研究计划(No.2014CB543102) （No.2014CB543102）
国家自然科学基金项目(No.81403487) （No.81403487）
湖南省教育厅青年基金(No.14B128) （No.14B128）
湖南中医药大学研究生创新课题(No.2016CX25) （No.2016CX25）
