针刺研究2017，Vol.42Issue(4)：321-326,6.DOI:10.13702/j.1000-0607.2017.04.007
捏脊对颈椎病大鼠椎间盘细胞外基质及代谢酶的影响
Effects of Chiropractics on Intervertebral Disk Extracell Matrix and Metabolic Enzymes in Rats with Cervical Spondylosis
摘要
Abstract
Objective To observe the effect of chiropractics on intervertebral disk extracell matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, cellular collagen type Ⅱ (COL-Ⅱ), tissue inhibitor 1 of metalloproteinase (TIMP-1) and extracellular matrix (ECM) in the rats with cervical spondylosis, so as to explore its mechanism underlying improvement of cervical spondylosis.Methods A total of 40 SD rats were randomized into a sham group, a model group, a chiropractics group and a mobic group, 8 rats in each one.The model was established by static-dynamic imbalance method.The rats in the chiropractics group were treated with chiropractic therapy for two courses, 14 times a day and 14 days as a course.The rats in the mobic group were treated with intragastric administration of mobic.The expression of MMP-1, MMP-3, MMP-13, COL-Ⅱ and TIMP-1 in the intervertebral disk tissue were detected by immunohistochemistry, and the protein expression of ECM was detected by Western blot.Results Compared with those in the sham group, the expressions of MMP-1, MMP-3, MMP-13 in the model group increased (all P<0.05), and COL-Ⅱ, TIMP-1 decreased (both P<0.05).Compared with those in the model group, the expressions of MMP-1, MMP-3, MMP-13 in the chiropractics group and the mobic group decreased (all P<0.05), and COL-Ⅱ, TIMP-1 increased (all P<0.05).Compared with that in the sham group, the expression of ECM protein in the model group decreased (P<0.05).Compared with that in the model group, the expression of ECM protein in the chiropractics group and the mobic group increased (both P<0.05).Conclusion Chiropractics may improve the degeneration in rats with cervical spondylosis by regulating intervertebral disc ECM system and relevant metabolic enzymes.关键词
捏脊/颈椎病/细胞外基质/Ⅱ型胶原Key words
Chiropractics/Cervical spondylosis/Extracellular matrix/Collagen type Ⅱ分类
医药卫生引用本文复制引用
黄黎珊,黄萍萍,童秀冰,李忠丽,方燕平,廖军..捏脊对颈椎病大鼠椎间盘细胞外基质及代谢酶的影响[J].针刺研究,2017,42(4):321-326,6.基金项目
国家自然科学基金项目(No.81273836、81001554) （No.81273836、81001554）
