中国病理生理杂志2017，Vol.33Issue(8)：1475-1480,6.DOI:10.3969/j.issn.1000-4718.2017.08.021
小窝蛋白1脚手架区多肽减轻LPS诱导的小鼠急性肺损伤
Effects of caveolin-1 scaffolding domain peptide on LPS-induced acute lung injury in mice
摘要
Abstract
AIM: To investigate the effects of caveolin-1 (Cav-1) scaffolding domain peptide, cavtratin, on lipopolysaccharide (LPS)-induced mouse acute lung injury and heme oxygenase-1 (HO-1) activity.METHODS: Adult male BALB/c mice were randomly divided into 6 groups (n=8 to 10): control, Antennapedia internalization sequence (AP), LPS, LPS+hemin, LPS+ hemin+cavtratin and LPS+hemin+cavtratin+zinc protoporphyrin IX (ZnPP) groups.After LPS administration for 24 h, the lung pathological changes, the wet/dry weight (W/D) ratio of lung tissues, total cell number in bronchoalveolar lavage fluid and serum lactate dehydrogenase activity were measured.The co-localization of HO-1 and Cav-1 was displayed by immunofluorescence, and the HO-1 activity were detected.The mRNA expression of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, MCP-1 and iNOS was detected by real-time PCR.RESULTS: The mice in LPS+hemin+cavtratin group had the decreased interaction between HO-1 and Cav-1, and the increased HO-1 activity compare with LPS group (P<0.05).Compared with LPS group, the pulmonary damage was attenuated in LPS+hemin+cavtratin group, and the injury indexes, including W/D ratio, total cell number in bronchoalveolar lavage fluid and lactate dehydrogenase activity in the serum, and the mRNA expression of inflammatory cytokines all decreased (P<0.05).HO-1 activity inhibitor ZnPP abolished the above protective effect of cavtratin on the lung tissues with LPS-induced acute lung injury.CONCLUSION: Cavtratin has beneficial effects on the lung with LPS-induced acute injury by restoring the HO-1 activity.关键词
小窝蛋白1/血红素加氧酶1/急性肺损伤/脂多糖Key words
Caveolin-1/Heme oxygenase-1/Acute lung injury/Lipopolysaccharide分类
医药卫生引用本文复制引用
翁平,张晓彤,陈炜,田文芳,陈俊良,苑佳佳,陈新杰,庞庆丰..小窝蛋白1脚手架区多肽减轻LPS诱导的小鼠急性肺损伤[J].中国病理生理杂志,2017,33(8):1475-1480,6.基金项目
国家自然科学基金资助项目(No.81270126) （No.81270126）
中央高校基本科研业务费专项资金资助(No.JUSRP51412B) （No.JUSRP51412B）
江苏省研究生培养创新工程项目(No.KYZZ16_0312 （）
No.KYLX15_1196) （）
国家级大学生创新创业训练计划项目(No.201610295074) （No.201610295074）
江苏省医学会/康缘药业临床医学科研专项基金(No.2015JZKY07) （No.2015JZKY07）
