中国病理生理杂志2017，Vol.33Issue(8)：1524-1527,4.DOI:10.3969/j.issn.1000-4718.2017.08.029
3-甲基腺嘌呤联合曲古霉素A抑制三阴性乳腺癌细胞生长
3-MA combined with TSA inhibits growth of triple-negative breast cancer cells
摘要
Abstract
AIM: To investigate the effect of 3-methyladenine (3-MA) combined with trichostatin A (TSA) on triple-negative breast cancer cells and the mechanism involved.METHODS: The viability of MDA-MB-231 cells was detected by MTT assay, the migration ability was determined by scratch assay, and the expression of autophagy-related proteins was detected by Western blot.RESULTS: TSA significantly inhibited the viability of MDA-MB-231 cells in a time-and dose-dependent manner.The results of scratch assay showed that TSA inhibited cell migration ability.Western blot data indicated that TSA resulted in a moderate increase in LC3-Ⅱ expression.Moreover, 3-MA inhibited cell autophagy induced by TSA, and combination of 3-MA and TSA further inhibited the viability of MDA-MB-231 cells.CONCLUSION: Combination of 3-MA and TSA may effectively inhibit the growth of triple-negative breast cancer cells.关键词
乳腺癌/曲古霉素A/3-甲基腺嘌呤/自噬Key words
Breast cancer/Trichostatin A/3-methyladenine/Autophagy分类
医药卫生引用本文复制引用
朱坤,徐明,丁米娜,林贞花,陈丽艳..3-甲基腺嘌呤联合曲古霉素A抑制三阴性乳腺癌细胞生长[J].中国病理生理杂志,2017,33(8):1524-1527,4.基金项目
国家自然科学基金资助项目(No.81460399) （No.81460399）
