中国动脉硬化杂志2017，Vol.25Issue(8)：773-777,5.
鸢尾素对载脂蛋白E基因敲除小鼠动脉粥样硬化的影响
The effect of irisin on atherosclerosis in apolipoprotein E gene knockout mice
摘要
Abstract
Aim To study the effect of irisin on the process of atherosclerosis in apolipoprotein E knockout (ApoE-/-) mice.Methods ApoE-/-mice were randomly divided into three groups:normol group,atherosclerosis model group and irisin group,each group contained 10 mice.ApoE-/-mice in normol group were fed with normal diet and saline,ApoE-/-mice in atherosclerosis model group were fed with high fat diet and saline,ApoE-/-mice in irisin group were fed with high fat diet and irisin,all mice were fed for 12 weeks.Total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDLC),high density lipoprotein cholesterol (HDLC),the serum levels of superoxide dis mutase (SOD) and malondialdehyde (MDA) were measured by chemical method,HE staining and total oil red O staining were used to analyze the size of aortic atherosclerotic plaque area,the mRNA and protein level of CD36,NF-κB p65,SOD were detected by real-time quantitative PCR (qPCR) and Western blot.Results The area of aortic plaque in atherosclerosis model group was significandy higher than that in normal group,the area of aorta plaque in irisin group was significantly lower than that in atherosclerosis model group.Compared with normal group,body weight of mice,TC,TG,LDLC,MDA,the expression of NF-κB p65 and CD36 mRNA and protein in atherosclerosis model group increased,while the activity of serum SOD and HDLC levels decreased (P＜0.05).Compared with atherosclerosis model group,body weight of mice,TC,TG,LDLC,MDA,the expression of NF-κB p65 and CD36 mRNA and protein in irisin group decreased,while the activity of serum SOD and HDLC levels increased (P＜0.05).Conclusion Irisin can reduce the occurrence and development of atherosclerosis in ApoE-/-mice,which may be related to its decreasing body weight and blood lipid,inhibition of inflammation and anti-oxidative stress.关键词
鸢尾素/载脂蛋白E基因敲除小鼠/动脉粥样硬化Key words
Irisin/Apolipoprotein E gene knockout mice/Atherosclerosis分类
医药卫生引用本文复制引用
谭娟,刘大男,何青松,肖金翠,钟志雄,莫显刚..鸢尾素对载脂蛋白E基因敲除小鼠动脉粥样硬化的影响[J].中国动脉硬化杂志,2017,25(8):773-777,5.基金项目
国家自然科学基金项目(81660083) （81660083）
贵州省高层次创新型人才培养计划项目[黔科合人才(2015) 4026号] （2015）
贵州省社会发展攻关项目[黔科合SY(2013)3017号] （2013）
贵州省优秀青年科技人才培养对象专项资金项目[黔科合人字(2011)26号] （2011）
贵州省对外合作项目[黔科合省合(2011)7001号] （2011）
