中国动脉硬化杂志2017，Vol.25Issue(8)：778-782,5.
ADAM17调控CGRP对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖的抑制效应
ADAM17 regulates inhibitory effect of CGRP on angiotensin Ⅱ-induced proliferation of vascular smooth muscle cells
摘要
Abstract
Aim To explore a disintergrin and metalloproteinase 17 (ADAM17) regulating the protective effect of calcitonin gene-related peptide (CGRP) against proliferation of vascular smooth muscle cell (VSMC) and its mechanism.Methods The rat thoracic aortic smooth muscle cell line (A10VSMC) was used as the object of study.RNA interference was adopted to reduce ADAM17 expression.Western blot and real-time quantitative PCR were used to detect the expression levels of protein and mRNA.Methylthiazolyldiphenyl-tetrazolium bromide assay was used to evaluate cell viability of VSMC.Results CGRP could markedly attenuate angiotensin Ⅱ-induced VSMC proliferation.There were significant increases in expression levels of ADAM17 protein and mRNA in VSMC stimulated with angiotensin Ⅱ for 24 hours.Pretreatment with CGRP for 30 minutes significantly reduced the expression levels of ADAM17 protein and mRNA,whereas the effects could be reversed by CGRP receptor antagonist CGRP8-37.The results of RNA interference showed that ADAM17 siRNA significantly inhibitted angiotensin Ⅱ-mediated VSMC proliferation.Conclusion The reduction of ADAM17 expression may be the mechanism by which CGRP inhibits angiotensin Ⅱ-mediated VSMC proliferation.关键词
肿瘤坏死因子α转化酶/降钙素基因相关肽/细胞增殖/血管平滑肌细胞/血管紧张素ⅡKey words
A disintergrin and metalloproteinase 17/Calcitonin gene-related peptide/Cell proliferation/Vascular smooth muscle cell/Angiotensin Ⅱ分类
医药卫生引用本文复制引用
曾泗宇,洪陈亮,兰树敏,卢慧勤,陈燕,严秋江,秦旭平..ADAM17调控CGRP对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖的抑制效应[J].中国动脉硬化杂志,2017,25(8):778-782,5.基金项目
广东省自然科学基金(2015A030310076) （2015A030310076）
广东省科技计划(2016A020226005) （2016A020226005）
广东省医学基金(A2014159) （A2014159）
