中国动脉硬化杂志2017，Vol.25Issue(8)：791-795,829,6.
间歇性碱刺激对高磷诱导的大鼠血管环钙化的影响及可能机制
Effect of intermittent alkaline stimulation on aortic ring calcification in rat induced by high phosphorus and its mechanism
摘要
Abstract
Aim To explore the effects of intermittent alkaline stimulation on vascular ring calcification from the thoracic aorta of rats induced by high phosphorus and the possible mechanism.Methods Aortic rings were isolated from rat thoracic aorta and cultured in vitro,and randomly divided into three groups:control group,high phosphorus group (containing 10 mmol/L β-glycerol phosphate medium) and intermittent alkaline stimulation group (adjusting pH to 7.7 on the basis of high phosphorus medium).After 14 days of intervention,the expressions of L-type calcium channels (LTCC) β3 subunit,runt-related transcription factor 2 (Runx2) and smooth muscle protein 22α (SM22α) were detected by immunohistochemical method.The degree of vascular ring calcification was detected by silver nitrate staining and calcium content test.Results Compared with control group,calcium content,the expressions of Runx2 and LTCCβ3 were significandy increased in high phosphorus group (P＜0.001).Compared with high phosphorus group,calcium content,the expressions of Runx2 and LTCCβ3 were significantly increased in intermittent alkaline stimulation group (P＜0.001).At the same time,the number of brown and black calcified nodules in high phosphorus group was higher than that in control group,and the number of brown and black calcified nodules in intermittent alkaline stimulation group was higher than that in high phosphorus group.Compared with control group,the expression of SM22α was significantly decreased in high phosphorus group (P＜0.001).Compared with high phosphorus group,the expression of SM22αt was significantly decreased in intermittent alkaline stimulation group (P＜0.001).Correlation analysis showed that the expression of LTCCβ3 was positively correlated with Runx2 protein expression (r =0.704,P =0.002) and negatively correlated with SM22α protein expression (r =-0.670,P =0.006).Conclusion Intermittent alkaline stimulation can promote high phosphorus induced vascular ring calcification from the thoracic aorta of rats.Its mechanism may be upregulation of LTCCβ3 protein expression,enhancing the transformation of vascular smooth muscle cells into osteogenesis/chondrogenesis phenotype,and then promoting the occurrence of vascular ring calcification.关键词
间歇性碱刺激/血管环钙化/L型钙通道β3亚基/血管平滑肌细胞/表型转化/维持性血液透析Key words
Intermittent alkaline stimulation/Aortic ring calcification/L-type calcium channels β3 subunit/Vascular smooth muscle cell/Phenotypic transformation/Maintenance hemodialysis分类
医药卫生引用本文复制引用
田恬,徐金升,白亚玲,张俊霞,崔立文,张胜雷..间歇性碱刺激对高磷诱导的大鼠血管环钙化的影响及可能机制[J].中国动脉硬化杂志,2017,25(8):791-795,829,6.基金项目
河北省自然科学基金项目(2012206157) （2012206157）
河北省科技计划项目(16397733D、20150351、20150310) （16397733D、20150351、20150310）
