中国动脉硬化杂志2017，Vol.25Issue(8)：812-817,6.
miR-4463在下肢动脉硬化闭塞症中的表达及意义
The expression of miR-4463 in patients with arteriosclerosis obliterans
摘要
Abstract
Aim To observe the expression of miR-4463 in the plasma and tissues of patients with arteriosclerosis obliterans of lower limbs (ASO).Methods miRNA microarray method was used to screen the differentially expressed miRNA in the plasma of three ASO patients and three controls.Real time PCR technique was carried out to verify the expression level of miR-4463 in the plasma and tissues of 50 patients with ASO and 50 healthy controls,and the relationship of clinical stage with miR-4463 level was analyzed.Target gene prediction software was used to predict target genes of miR4463,and then the function and signaling pathways of predicted target genes were analyzed by Gene Ontology and KEGG pathway.Results Compared with the controls,51 miRNAs changed in the plasma of patients with ASO more than 1.5 times (P＜0.05).The expression of miR-4463 in plasma and vascular intimal tissues of ASO patients was significantly decreased,and decreased gradually with Fontaine stage.Bioinformatics analysis revealed that the target genes of miR-4463 were related to cell polarity,migration,lipid metabolism and endocytosis.Conclusion miR-4463 is involved in the pathogenesis of ASO,and decreased miR-4463 level may indicate the occurrence of ASO.关键词
miR-4463/下肢动脉硬化闭塞症/miRNA芯片方法Key words
miR-4463/Arteriosclerosis obliterans/miRNA microarray method分类
医药卫生引用本文复制引用
何雪梅,王雪琴,杜超,陈俊,梁冠舅,龙洋,周翔宇..miR-4463在下肢动脉硬化闭塞症中的表达及意义[J].中国动脉硬化杂志,2017,25(8):812-817,6.基金项目
四川省教育厅科研项目(16ZA0179) （16ZA0179）
泸州市-泸州医学院联合课题(LZ-LY-20) （LZ-LY-20）
