中国感染控制杂志2017，Vol.16Issue(8)：693-697,5.DOI:10.3969/j.issn.1671-9638.2017.08.001
患者及相关环境分离CRKP的耐药谱与同源性
Antimicrobial resistance profile and homology of carbapenem-resistant Klebsiella pneumoniae isolated from patients and related surroundings
摘要
Abstract
Objective To understand the homology of clinical isolates from patients with carbapenem-resistant Klebsiella pneumoniae(CRKP)infection and isolates from environment in a medical institution.Methods One CRKP strain isolated from a patient in this hospital and 4 strains of Klebsiella pneumoniae(K.pneumoniae)isolated from patient's surroundings were collected,susceptibility of 5 strains to commonly used antimicrobial agents was detected,production of carbapenems in 5 strains were detected by modified Hodge testing and carbapenem inactivation method(CIM),homology analysis was performed by pulsed-field gel electrophoresis(PFGE).Results Antimicrobial susceptibility testing results showed that 5 strains of K.pneumoniae(1 from patient,4 from the patient's ward surroundings,including hands of nursing aides,solution bottle opening,handle for lifting and dropping bed,and bedrail)were all resistant to other antimicrobial agents except to cephamycin and aminoglycosides.The modified Hodge testing and CIM confirmed that 5 strains all produced carbapenemases;PFGE results showed that electrophoretogram of CRKP isolated from solution bottle opening of ward,bedrail,and handle for lifting and dropping bed were the same as CRKP isolated from patient,while electrophoretogram of CRKP isolated from hands of nursing aides had 2 different bands,there was a close relationship between the strains.Conclusion The same type of CRKP were isolated from patient and his surroundings,it is necessary to implement healthcare-associated infection(HAI)control system,isolate infected patient,and strengthen environmental cleaning and disinfection,so as to avoid the outbreak of HAI.关键词
肺炎克雷伯菌/碳青霉烯酶类抗生素/脉冲场凝胶电泳/同源性分析Key words
Klebsiella pneumoniae/carbapenems/pulsed-field gel electrophoresis/homology analysis分类
医药卫生引用本文复制引用
杨竹兰,张震,刘智勇,吴昊,张波..患者及相关环境分离CRKP的耐药谱与同源性[J].中国感染控制杂志,2017,16(8):693-697,5.基金项目
国家自然科学基金项目(No.71373280) （No.71373280）
第三军医大学临床创新基金项目(SWH2013LC08,SWH2014LC31) （SWH2013LC08,SWH2014LC31）
