中国感染控制杂志2017，Vol.16Issue(8)：698-701,4.DOI:10.3969/j.issn.1671-9638.2017.08.002
三种用于血液透析和相关治疗用水微生物学监测方法的评价
Three methods for microbiological monitoring on dialysate and dialysis water for hemodialysis
摘要
Abstract
Objective To evaluate detection effect of three methods on monitoring microbes in dialysate and dialysis water for hemodialysis.Methods Seventy-two dialysate and dialysis water specimens were collected from 36 medical institutions,specimens were cultured with three methods: blood agar plate incubated at 35℃ for 72 hours,Tryptic soy agar(TSA)plate incubated at 35℃ for 72 hours,and Reasoner's 2A agar(R2A agar)plate incubated at 23℃ for 168 hours,colony counts,isolation of colony,and detection rate of colony exceeding action level(≥50 CFU/mL)were compared among three methods.Results The colony isolation rates of microbes in dialysate and dialysis water detected by blood agar plate,TSA plate and R2A plate were 40.28%,63.89%,and 69.44%respectively,difference was significant(x2=14.16,P<0.05);pairwise comparison showed that isolation rates of colony on R2A agar plate and TSA plate were higher than blood agar plate.There was significant difference in isolated colony count between blood agar plate and R2A agar plate,TSA plate and R2A agar plate respectively(Z=-4.515,-6.970 respectively,both P<0.05).The rates of isolated colony exceeding action level in dialysate and dialysis water detected by blood agar plate,TSA plate,and R2A agar plate were 1.39%,4.17%,and 20.83%respectively,difference was significant(x2=19.83,P<0.05),detection rate of R2A agar plate was higher than the other two methods.Conclusion The detection rate of colony by R2A agar plate and TSA plate are better than blood agar plate,detection rate of colony exceeding action level by R2A agar plate is higher than TSA plate and blood agar plate,R2A agar plate for microbial monitoring(23℃,168 h)on dialysate and dialysis water is superior to the other two methods.关键词
血液透析/透析液/透析用水/微生物学监测Key words
hemodialysis/dialysate/dialysis water/microbiological monitoring分类
医药卫生引用本文复制引用
钱雪峰,乔美珍,金美娟,马文霞,杨波..三种用于血液透析和相关治疗用水微生物学监测方法的评价[J].中国感染控制杂志,2017,16(8):698-701,4.基金项目
国家自然科学基金(81501425) （81501425）
