中国临床药理学杂志2017，Vol.33Issue(13)：1233-1236,4.DOI:10.13699/j.cnki.1001-6821.2017.13.018
红芪多糖对免疫抑制大鼠免疫功能与细胞因子的影响
Effect of Radix hedysari polysaccharide on the immune function and cytokines of immune suppression induced by cyclophosphamide in rat
摘要
Abstract
Objective To observe the effect of Radix hedysaxi polysaccharide(RHP) on the immune function and cytokines of immune suppression induced by cyclophosphamide in rats.Methods Sixty SD rats were treated with intraperitoneal injection of cyclophosphamide (CTX),each time 80 mg · kg-1,1 times/day,with 3 d.After the success of the model group intervention treatment,the rats was divided into 3 groups:blank group,model group and experimental group.The rats in experimental group was treated with the dose of 100 mg · kg-1 · d-1 RHP,and the rats in the blank group and the model group were fed with distilled water of equal volume,1 times/day,administrating 7 d.Drug delivery in 7 days,rats in groups fasting 8 hours,femoral artery blood.The serum levels of immunoglobulin IgG,IgM and IgA were detected by enzyme league immune method.The serum content of immune cell factor of tumor necrosis factorα (TNF-α),interferon-γ(IFN-γ),interleukin-2(IL-2)and IL-4 were detected by enzyme league immune method.Results After administration,IgG in blank group,model group and experimental group were (0.98 ± 0.50),(0.28 ± 0.18),(0.60 ± 0.28) μg · mL-1;IgM in the three groups were (365.21 ± 26.85),(267.03 ± 47.89),(286.22 ± 30.03)μg · mL-1;IgA in the three groups were (79.52 ±9.30),(57.88 ± 10.44),(6 9.19 ± 14.18) μg · mL-1.Compared with blank group,the difference in model group was statistically significant (all P ＜ 0.05).Compared with model group,the difference in experimental group was statistically significant (all P ＜ 0.05).After administration,TNF-α in blank group,model group and experimental group were (39.52 ± 5.28),(70.14 ± 6.68),(51.16 ± 5.60)pg · mL-1;IFN-γin the three groups were (24.31 ± 5.86),(10.88 ± 3.17),(15.52 ± 3.92) pg · mL-1;IL-2 in the three groups were (261.51 ±46.20),(153.7 ±34.96),(185.84 ± 17.74) pg · mL-1;IL-4 in the three groups were (18.91 ± 2.56),(9.32 ± 3.86),(15.23 ± 2.75) pg · mL-1.Compared with blank group,the difference in model group was statistically significant (all P ＜ 0.05).Compared with model group,the difference in experimental group was statistically significant (all P ＜ 0.05).Conclusion RHP can improve the immunosuppression caused by cyclophosphamide model immune function in rats.关键词
红芪多糖/免疫抑制/免疫功能/细胞因子Key words
Radix hedysari polysaccharide/immunosuppression/immunoglobulin/cytokines分类
医药卫生引用本文复制引用
颜春鲁,李钦,姚贞宇,刘永琦..红芪多糖对免疫抑制大鼠免疫功能与细胞因子的影响[J].中国临床药理学杂志,2017,33(13):1233-1236,4.基金项目
甘肃省中医药管理局科研基金资助项目(GZK-2015-45) （GZK-2015-45）
