中国临床药理学杂志2017，Vol.33Issue(14)：1317-1320,4.DOI:10.13699/j.cnki.1001-6821.2017.14.009
Toll样受体4/核转录因子kappa B信号通路及其相关炎症因子对周围性面神经炎的影响
Effect of toll-like receptor 4 / nuclear factor-kappa B pathway and its related inflammatory markers on peripheral facial neuritis
摘要
Abstract
Objective To explore the effect of toll-like receptor 4 (TLR 4) / nuclear factor-kappa B (NF-κB) pathway and its related inflammatory markers on peripheral facial neuritis.Methods Fifty patients with peripheral facial neuritis were enrolled in the treatment group,and fifty healthy subjects were enrolled in the control group.Real-time fluorescent quantitative PCR (RT-qPCR) and Western blot was used to detect the expression of TLR4 mRNA and protein.Western blot were used to detect NF-κB p65 phosphorylation in peripheral blood mononuclear cell (PBMCs).Enzyme linked immunosorbent assay (ELISA) was used to detect the content of serum tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β).Results The relative expression levels of TLR4 mRNA in PBMCs of treatment group and control group were 1.55 ± 0.25 and 0.83 ±0.10,while that of TLR4 protein were 1.11 ±0.17,0.61 ±0.11;the phosphorylation levels of NF-κB p65 in treatment group and control group were 0.67 ± 0.30,0.36 ±± 0.22;the serum TNF-α in the treatment group and the control group were (149.50±12.12),(102.10±10.60) ng L-1,while the serum IL-1β3 were (255.50 ± 19.19),(105.20 ± 12.80)ng · L-1,the differences were statistically significant (P ＜0.05).Conclusion The expression changes of TLR4/NF-κB pathway in peripheral blood mononuclear cells and related in flammatory markers in serum may play a role in peripheral facial neuritis.关键词
周围性面神经炎/外周血单个核细胞/Toll样受体4/核转录因子kappa B信号通路/炎症因子Key words
peripheral facial neuritis/peripheral blood mononuclear cells/toll-like receptor 4 / nuclear factor-kappa B pathway/inflammatory factor分类
医药卫生引用本文复制引用
颜建辉,黄丽娟,刘剑萍,欧阳慧,罗高梅,王文婷,陈雁斌..Toll样受体4/核转录因子kappa B信号通路及其相关炎症因子对周围性面神经炎的影响[J].中国临床药理学杂志,2017,33(14):1317-1320,4.基金项目
湖南省教育厅科技基金资助项目(15C1282 （）
448) （）
